GgaEX0019786 @ galGal4
Exon Skipping
Gene
ENSGALG00000013124 | FHOD3
Description
formin homology 2 domain containing 3 [Source:HGNC Symbol;Acc:HGNC:26178]
Coordinates
chr2:83359162-83389727:+
Coord C1 exon
chr2:83359162-83359400
Coord A exon
chr2:83378619-83378744
Coord C2 exon
chr2:83389593-83389727
Length
126 bp
Sequences
Splice sites
3' ss Seq
TATACTCCTCACTTCTTCAGATA
3' ss Score
8.91
5' ss Seq
CAGGTTTGT
5' ss Score
7.44
Exon sequences
Seq C1 exon
ATGACCCTGAGGCACGAAGATGGCGATGAGACCGCTGACCCCCCTCCCAGTGGGCGCAAGGACAGAAGAAGGGCCAGTCTGGGTGGCAGTGAGCGAAGGGGTCTGGAGCGCCGACGGAGCCGCAGGCACTCAGTACAGAACGTCAAAAGCACTTTATCAGCTCCCGCCAGTCCATGCAGTCAGTCAAACCCCAGCTTCATGCTAGGCCACGGACAGCGCGTGGATGATCTCAGTGAGAG
Seq A exon
ATACAATAGCTATGGCAGCAACTCTTACTCTTCTCCGCGACCTTACTCTGGTTTGAGTGCTCCTACAACACCCACTTCTCGTTATGGGACAACTCTCTCACCACCTCAGGAGTCCAAGTCTGACAG
Seq C2 exon
GCCTGCTTTGGGTGGTCTTCTTACATCATCTTTCCGGCAGCACCAGGAGTCCTTGGCAGCAGAAAGAGAAAGACGGCGCCAAGAGAGAGAAGAAAGGTTGCAGAGGATAGAACGTGAAGAAAGAAACAAATTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000013124_MULTIEX1-4/4=C1-C2
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.984 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGACAGAAGAAGGGCCAGT
R:
CTTTCTGCTGCCAAGGACTCC
Band lengths:
248-374
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]