RnoEX0036008 @ rn6
Exon Skipping
Gene
ENSRNOG00000027230 | Fhod3
Description
formin homology 2 domain containing 3 [Source:RGD Symbol;Acc:2322319]
Coordinates
chr18:17218299-17254858:+
Coord C1 exon
chr18:17218299-17218655
Coord A exon
chr18:17224244-17224348
Coord C2 exon
chr18:17254724-17254858
Length
105 bp
Sequences
Splice sites
3' ss Seq
CCAACCTCCGTGCCTTTTAGATA
3' ss Score
8.69
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
Exon sequences
Seq C1 exon
CAAAGCCAGCTCTGCCTCCAAGCAGAGCCCTCCTGAGAAGGATGCTACTCCAGAGAGTTCTACACTCCGTACCACCTCAAGTCCCACCTCACAGGGAAGGTGGCTTCCGGCCAACCCAGCAGCACAGAGCCCGGTGCTTGCTGGGACCTCTGGGCCTGAGGCCAGCCGGCCTGCCGCTCGCTTACCACCTCCCAGCCCTGTCTTGGCTCCTCGGCCGTCCACCGCACCAAAGGTGTCTCCCACCATAGACAAGCTGCCCTACGTGCCCCACAGCCCCTTCCACCTCTTCTCCTATGACTTTGAAGATTCACCTCTCCTCACTAAGGACAAGGGAGGAGACTCTCAGACGGAAAACAG
Seq A exon
ATACAGCAACTTCAGCAGCAGCTCTTTCCAGTCCTCCAGACCCTCTCCTGGACCTAGTGTGTCTCCATCTTCCGCATCGTCCTTCTCATCCCCACAGGATACCAG
Seq C2 exon
GTCATCGCCAAGTGGCCTTCTCACATCCTCCTTTAGACAGCACCAAGAGTCACTGGCAGCGGAGAGAGAGCGGAGGCGGCAAGAGAGAGAAGAAAGACTGCAGAGGATAGAACGGGAAGAAAGGAACAAATTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000027230-'40-34,'40-31,41-34
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.925 A=0.983 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTCTCCCACCATAGACAAGCT
R:
TTCCCGTTCTATCCTCTGCAGT
Band lengths:
242-347
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]