BtaEX6091523 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021581 | FHOD3
Description
formin homology 2 domain containing 3 [Source:HGNC Symbol;Acc:HGNC:26178]
Coordinates
chr24:20757661-20812841:-
Coord C1 exon
chr24:20812600-20812841
Coord A exon
chr24:20784954-20785067
Coord C2 exon
chr24:20757661-20757795
Length
114 bp
Sequences
Splice sites
3' ss Seq
TAACTCTCTGTACTCTTCAGATA
3' ss Score
8.1
5' ss Seq
AAGGTGGGT
5' ss Score
8.23
Exon sequences
Seq C1 exon
GTGGCGCTCCGGCTGGAGGATGGGGATGAGACGGCAGAGCCGCCCCCCAGCGGGTGCCGTGACCGCAGGCGGGCCAGTGTGTGCTCCGGAGGCACGGGCAGTGAGCCCCGGGGCCTGGACCGAAGGCGGAGCCGCAGGCACTCGGTGCAGAACATCAAGAGCCCCCTGTCGCCCCCGTCCAGCCCCTGCTCCCTGTCGGCTCCTGACTTCAAGCCCAGTCAAGTGCGAGATCTCCGGGAAAA
Seq A exon
ATACAGCAGCTTTGGCAACAACTCTCATCACTCCTCGAGACCTTCACCTGGATCCAGTGTGCCCACCACCCCCACGTCATCCGTCTCACCCCCACAGGAGGCCAGGCCTGAAAG
Seq C2 exon
GTCATCAGTGAGTGGTCTTCTCACGTCATCCTTTAGACAGCACCAAGAGTCCTTGGCAGCAGAGAGAGAGAGGCGGCGACAGGAGAGAGAGGAAAGGTTGCAGAGAATAGAACGAGAGGAAAGAAACAAATTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021581-'18-23,'18-22,23-23=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.960 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGGGGATGAGACGGCAGAG
R:
AGGATGACGTGAGAAGACCACT
Band lengths:
256-370
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]