GgaEX1056945 @ galGal4
Exon Skipping
Gene
ENSGALG00000013370 | Sema3F
Description
semaphorin-3F precursor [Source:RefSeq peptide;Acc:NP_989589]
Coordinates
chr12:3181974-3183327:+
Coord C1 exon
chr12:3181974-3182093
Coord A exon
chr12:3182991-3183130
Coord C2 exon
chr12:3183213-3183327
Length
140 bp
Sequences
Splice sites
3' ss Seq
ACTGCGTCGTGCCCTTGCAGACC
3' ss Score
10.39
5' ss Seq
CTGGTGTGT
5' ss Score
4.96
Exon sequences
Seq C1 exon
ATGAAGAACTCTATGCTGGGGTCTACATCGACTTCATGGGCACTGATGCAGCCATCTTCCGCACTATGGGCAAGCAGACAGCCATGAGGACAGACCAGTACAATTCACGCTGGCTCAACG
Seq A exon
ACCCTGCCTTCGTCCGTGCCCAGCTCATCCCTGACAGCAGCGAGAGGAATGATGACAAACTCTACTTCTTCTTCCGGGAGAAGTCGGCGGATGCCCCGCTCAGCCCCGGGGTCTATTCCCGCATCGGGCGCATCTGCCTG
Seq C2 exon
AACGACGATGGAGGACACTGCTGCCTGGTGAACAAATGGAGCACCTTCCTGAAGGCCCGGCTCGTCTGCTCCGTGCCAGGACCCGATGGGATCGAGACACACTTTGATGAGCTTC
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000013370-'13-19,'13-18,14-19
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=0.007 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(9.0=100)
A:
PF0140314=Sema=FE(10.4=100)
C2:
PF0140314=Sema=FE(8.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAAGAACTCTATGCTGGGGTCT
R:
GAAGCTCATCAAAGTGTGTCTCGA
Band lengths:
234-374
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]