MmuEX6068700 @ mm10
Exon Skipping
Gene
ENSMUSG00000034684 | Sema3f
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F [Source:MGI Symbol;Acc:MGI:1096347]
Coordinates
chr9:107687730-107688349:-
Coord C1 exon
chr9:107688230-107688349
Coord A exon
chr9:107687949-107688088
Coord C2 exon
chr9:107687730-107687844
Length
140 bp
Sequences
Splice sites
3' ss Seq
CCACCTCCCCCCCCTTCCAGATC
3' ss Score
12.58
5' ss Seq
CTCGTAAGT
5' ss Score
9.63
Exon sequences
Seq C1 exon
ATGAGGAACTCTATGCAGGAGTGTACATCGATTTTATGGGTACCGATGCAGCCATTTTCCGCACGCTGGGGAAGCAGACAGCCATGCGCACAGATCAGTACAACTCCCGGTGGCTCAATG
Seq A exon
ATCCTTCCTTCATACATGCTGAGCTCATCCCTGACAGTGCAGAGCGCAATGACGATAAACTCTACTTCTTCTTCCGAGAGCGCTCGGCAGAGGCGCCACAGAACCCTGCTGTGTATGCCCGCATCGGGCGCATCTGCCTC
Seq C2 exon
AACGATGACGGTGGCCATTGCTGCCTGGTCAACAAGTGGAGCACATTCCTGAAGGCTCGGCTCGTCTGCTCCGTGCCAGGCGAGGATGGTATTGAGACCCACTTCGACGAACTCC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034684-'28-25,'28-24,29-25=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.072 A=0.122 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(9.0=100)
A:
PF0140314=Sema=FE(10.4=100)
C2:
PF0140314=Sema=FE(8.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGAACTCTATGCAGGAGTGT
R:
AGTTCGTCGAAGTGGGTCTCA
Band lengths:
230-370
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types