GgaINT0010714 @ galGal4
Intron Retention
Gene
ENSGALG00000003157 | MYH7B
Description
myosin-7B [Source:RefSeq peptide;Acc:NP_989918]
Coordinates
chr20:2950255-2951294:-
Coord C1 exon
chr20:2951039-2951294
Coord A exon
chr20:2950498-2951038
Coord C2 exon
chr20:2950255-2950497
Length
541 bp
Sequences
Splice sites
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
3' ss Seq
CTTTCCTGTGTCTTTCTTAGGAG
3' ss Score
11.77
Exon sequences
Seq C1 exon
GGAAGCCCTCTATACCATCCAGTGGAACATCCGAGCCTTCAATGCTGTTAAGAACTGGTCCTGGATGAAACTGTTCTTCAAGATCAAGCCTTTGCTGAAGTCTGCTCAGACTGAGAAGGAAATGTCCACCTTGAAGGAAGAGTTCCAGAAGCTGAAGGAGGCTCTGGAGAAGTCGGAGGCTAAGAGGAAGGAGCTGGAAGAGAAGCAGGTCTCCATGATCCAGGAGAAGAATGACCTGGCTCTCCAGCTACAGGCA
Seq A exon
GTGAGTGCCTCACCCTCTCCCTGGCAGCATAAACATAGTGCAGGGTCCATGGATAAAGGAAGAAGCAGAGAGTGCTAGATCCCTTCTTCAGACTCTTGCAGTTCTGAGACCCACACATTGCCACGATCCATTTGAAATACAGTGTTAGACTCGGTGAAAACCTGAAGCTGCATTATAGGCTTGTTGTCTCAGGGCTGCCATGGGAATTCGTGGTTCCCTTGCTCTGAAAAAGGTGATCTCACATAAAGGTGGAAGTAATGGGAATGTTTGCTGTCCAAGCTGTGGCTGATCTGTGGCAATTCTGTGCCAGGAAGTGTCAGTTCAGTGTTTCTTGCCTCTGGAAAGAGAGTTGATGCAGTTGACTCTTTGGCTCTGGTCAGTGCTAGTTGTTGTCCCATGGCCCTGCATGGGGCTCCTTTCATTACACCTGATCACTTTGGGTTTGCTCTGTCTTCCACTCCTTGACTTTTGCAAGGACAGTGTGACTGCTGTATGCTTTTGGGGCAGTGGATGAGCACTGGCTTTCCTGTGTCTTTCTTAG
Seq C2 exon
GAGCAAGACAACCTGGCAGATGCAGAGGAACGCTGTGACCTTCTGATCAAGTCCAAGATCCAGCTGGAGGCCAAGGTGAAGGAGCTGACAGAGCGTGTGGAGGACGAGGAGGAGATGAACGCAGACCTCACTGCCAAGAAACGCAAGCTGGAGGATGAGTGTGCAGAGCTGAAGAAAGACATCGATGATCTAGAGATCACACTGGCCAAGGTGGAAAAGGAAAAGCATGCCACAGAGAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003157:ENSGALT00000012499:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.035 A=NA C2=0.247
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PU(27.1=60.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(41.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTGGAACATCCGAGCCTTC
R:
CGTTCATCTCCTCCTCGTCCT
Band lengths:
358-899
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]