GgaINT0079327 @ galGal4
Intron Retention
Gene
ENSGALG00000016289 | DST
Description
dystonin [Source:HGNC Symbol;Acc:HGNC:1090]
Coordinates
chr3:86482638-86483371:+
Coord C1 exon
chr3:86482638-86482797
Coord A exon
chr3:86482798-86483044
Coord C2 exon
chr3:86483045-86483371
Length
247 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAGA
5' ss Score
6.91
3' ss Seq
CTTCATTATTTAAAAAATAGGCC
3' ss Score
3.96
Exon sequences
Seq C1 exon
GAACCGTCAGTTGGAAGGCATCTCAGTGGTAGCACAGCAGTTTCATGAAGCTCTGGAGCCACTGGTGGAGTGGCTGTCGGCCACAGAGAAGAGACTTGCAAATGCTGAACCCATTGGAACACAGGCTTCCAAACTGCAACAGCAGATTTCTCAGCATAAA
Seq A exon
GTAAGATATGAACAACACATCTGCTCATGCCATTCTGTTTAAACTCATCTGTGAAGTAAGCACAATTTGGAAAGCTGCTGTGGTGCACTTGTATTTTACCATACTGATTTTTTTGCTGTAATTTGAGTTTTATTCATTTGTGTTGGCAATACATTCTTTTTAATTCAGTTTTTATTTAGTTCCACTTACATTTCTTTTATGTTTTTCTGATTTTCTTTTCCACTGTTCTTCATTATTTAAAAAATAG
Seq C2 exon
GCCCTAGAAGATGATGTCCTAGCTCACAATAAGAGTTTGCTTCAGGCCATTAGCATTGGTCAGTCTCTAAAGACTATGAGCTCCAGGGAAGATAAAGATATGGTGCAGGAAAAGCTAGATTCTTCTCAGGCCCGATACATTGAGATTCAAGAGAAGAGCAACAGCAGGTCTGAACTTCTACAGCAAGCGTACTGCAATGCTCAGATTTTTGGGGAGGACGAAGTTGAGTTGATGAACTGGCTGAATGAAGTCCATGATAAACTGAGCAAGTTGTCAGTCCAAGATTGCAACACAGAATTGCTTGAGAAACAGCACTCTGAACTACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016289:ENSGALT00000026264:70
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.148 A=NA C2=0.073
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(40.6=79.6)
A:
NA
C2:
PF0043516=Spectrin=PD(57.5=56.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCAAATGCTGAACCCATTGG
R:
ACTCAACTTCGTCCTCCCCAA
Band lengths:
293-540
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]