GgaINT0134958 @ galGal4
Intron Retention
Gene
ENSGALG00000001040 | MEGF6
Description
multiple EGF-like-domains 6 [Source:HGNC Symbol;Acc:HGNC:3232]
Coordinates
chr21:1014230-1015820:+
Coord C1 exon
chr21:1014230-1014352
Coord A exon
chr21:1014353-1015682
Coord C2 exon
chr21:1015683-1015820
Length
1330 bp
Sequences
Splice sites
5' ss Seq
ACCGTGAGT
5' ss Score
9.4
3' ss Seq
TGATGGAATTCCTTCTGCAGGGA
3' ss Score
7.64
Exon sequences
Seq C1 exon
ATGTGGATGAGTGCTTGGCCGGCCTGGCCACGTGTGCACACCGCTGCCTGAACACCCACGGCTCCTTTATGTGTGCCTGCAACCCGGGCTATGAGCTGGGAGCGGACAGCAGGCAGTGCTACC
Seq A exon
GTGAGTACCGCAGTGGGATGGGGGGGTCTGTGGGTCAGAAGGGGGGAAGAGGAGTGGAGGGGTCCTGCTGATGGAGAGCACTTGGTACTGCCTGTTGAGAGCAGACCGAGTCACAGCACTGTGGGCCATGGTCAGAGCTGTCAGCTTCTCCCACCAGCAGAACTCATCCTCCTCTTCAAAGTCACCATCGCACTGGTTTAGATGGTTTACTTGGAGCTGTTTGGCTCCAATTGGGCCAAGTCTCACTCTTGCATTGGATGAGTTTGGTCAGTTGTAGACGTGTAACTGAAGGAGGAATTGAGCTAATGATGTGATCACATCTGCTCCCATCAGGAAGAGAAATAAATACAATCCAATGCAAGAGTGAGCCAGATTGCCATATGTTCAGCCAAAGCACATCAGCAGAGACGGCCTGAAGCAAAGCAGTTCAGAGCAATGCAAAGAGCTGCGTGCTATTCAGGAGCCAGAGCACTGCTGGCACCTCTCATTCCTAAGGGCACGGCTGATCCCACGCTGCATGTTCTGCTGAACAAAATCCCACAGCTCGGTTTCGGTAGCCTCCACCTTTGCCTGTTGGGTGTTCTTGGATGAGCACACGCTTTTAGCATGTTTGTTCCCTGCTGTGCCCTGGCTGACCACACGTTAGCAGGGAAGGAGAGAACGTCCATGTTTTGAGATCTGGAAGTTAGCTTTCCGCCGTGGCACAGCTTTTGTTTTCTACATGCACAGACCCACTGGGGAGCACCGAGCTCCTCTTCTTTCATCTTTCTTGGGCCTGAATGCACCAGAACCTCAAGAACGTTCTTGTAGGCGGCAGCTATTTTTCTGAGGTTGTCAGTGACTCAGAGCCCCGTGGTAATTTTAGCCTAAAGAAGCTTCATTAGCTAGGCTGGAGTTTACAAGAGCTTTGCCAAGCTATGAAGACACATGGCTGCTATTGATGCTTGCCTCTTCCATTCGGACATGTCAGTCACTGCCTTTCCTAGCTGCAGGCATCTGGATGTTCGCAGCGCTCAGCTGCTCCCATTCCCAGCTGGCATCCAAAGCTCTGTGCTGGGAAGGCTGAGCCAACTCTGGCTGGCAGCACATCCTGCAGCTTGTCAGGAATGGGAGCAGCTCTGTGTGCAGTCTGGTTCAGCTCAGCTTGACCATGAGCCTGTCCCCGAGGGGCTCCACAGGGTTATTTTTGTGCAGCCCTTCCCAGCCCAGCATCTATTCCCATCCCTTCCCTGCAGCACAGCACTGCTCTGCTCAGCAGGGCCGTGTACCAAAAGCACCCACGAGGGGTGGGTGCCCGTGGCACTGGATGCTGATGGAATTCCTTCTGCAG
Seq C2 exon
GGATAGAAATGGAGATTGTGAGCAGCTGTGAGAGTGGCAACGGTGGCTGCTCCCATTTGTGCCACCACAGCAGCGCCGGCCCCATCTGCACCTGTAACTCAGGCTACCAGCTGCAGGATGACCAGAGGACATGCACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000001040:ENSGALT00000001544:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=95.2)
A:
NA
C2:
PF146701=FXa_inhibition=WD(100=76.6),PF0764510=EGF_CA=PU(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTGGATGAGTGCTTGGCC
R:
ATGTCCTCTGGTCATCCTGCA
Band lengths:
255-1585
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]