GgaINT1017727 @ galGal4
Intron Retention
Gene
ENSGALG00000014699 | MEGF10
Description
multiple EGF-like-domains 10 [Source:HGNC Symbol;Acc:HGNC:29634]
Coordinates
chrZ:56264105-56265721:+
Coord C1 exon
chrZ:56264105-56264341
Coord A exon
chrZ:56264342-56265593
Coord C2 exon
chrZ:56265594-56265721
Length
1252 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
ATCTTTCTTTTCCTTTTCAGAAA
3' ss Score
12.19
Exon sequences
Seq C1 exon
CTGGCGTAATTATAGTGGGAAACCTGAACAGCTTAAGCCGTACCAGTACTGCCATCCCTGCTGACTCTTACCAGATAGGGGCTATAGCAGGCATCATCATTCTTGTCTTGATTGTGCTCTTCCTGCTAGTGCTGTTCATTATTTACAGACATAAGCAAAAAGGGAAAGAAACAAACATGCCTGCAGTGACCTATACTCCTGCGATGAGGGTCATCAATGCTGATTACACCATTTCAG
Seq A exon
GTAAGTGAGATTTGGAATAGATTTTTCCCTCTGTCTCTTGACTTTGAGTTTTCAGCCAAGTTCTGAAGGGGTTCAGATCTGCTCTTAAAAGGGTTTCAATATGCATCTCATCTTTTTGCATCAGTGAGGGCTGCAGAGAGCAGGGAACTATTTGTTCCCAGAGAGTGTCTGAAAGTCTGCCTTGGAAGCCTACCTAGAAAGACAGACTGGGAAGGGTTTCCTTCTTTGGGAAGAGAGAGATTGCAATTCACTGCCCCCTATGGCCTTTTGCCAGCAGAGCAGCACCTAGGATGACATGCTGCTGGCAGATCAGAACACGGGAAAACTGGAATGCAGTGGTAGGCTATGAGACCTTCCTGCTGCTGCCAGAGATGGGGTTCGGAGGGAGCATGGCCCAGCATTTGCAGCCATTTAATTTGTTGTGTATCACAGTCTTTCAATCTGTGCATTTAGTACTAGAAAGCTGGCATACATTGGTAAAATACATTAATTACTGAATGAAGGATCAAGTGACCATATACCTTGCTTTACTGTAGAATGATTTTCTGTGTTAACCAGTGGAAGTATAGGTCTGTGCTTTAAAGAGACTTGGAAAGTGTGGCAAAACATGGTTTCTGCTAAGACATTAAATTTGATGCCAATGGCTAGAATATGGAAAGTCTGCTGTAGCTAGCTGTGGTTCTCTAGAGAGGCAGAGAACAGAGCTGCTCAAGTTTGAGACAAATGAAGGGAAGGAGCAATGCTTCTGAAACAGAATGGGAGTTTTCAAGCTGTGAAAGTCCACATAAGTTGGCATTAAATGGATAAATGAAAATGCAGGCAGTGCTGTCTTTTGCAAATTACTGTAAAGGAAATTGGCAGGTAAAGAAGCCAGCAAAAGCAGTTGTCAGAGTAGAGAAGGATGTACCACCACAAGTAATGGTGCAGTATAAGCACCAAAAAAATATACGGTGCTGTGAAGTCTAAGGTAAAAGTGTCCATAATTAGGACAGTGCCGTGATTACAGTTGCAAAAGTGAGCAGGGGACCACATCTGAAAGACAGGTCAGCTCGTAACATTTGAGAATGCTCTCTGGGGAAGGAAGGACACAGCTGGTCACTTACTGCTCTGTATCTCTAATTTATTCAGAGGAATGCCATATTTTTATTCAAGAAATGCTAGACTAGTCTGAAATCAGTGCAGTACTATGAAAAATAAGAAAGATACGATGCATTATTTTAAGTTGTTCATTTATCTTTCTTTTCCTTTTCAG
Seq C2 exon
AAACCATCCCTCATAGTAATGGTGGAAATGCTAACAGTCACTATTTCTCTAACCCCAGTTATCATACTCTAACACAGTGCACTACACCACCTCATGTCAACAACATGGACAGGCTGACTCTGGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000014699:ENSGALT00000023694:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.140
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(18.9=8.8),PF061436=Baculo_11_kDa=WD(100=51.2)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCTGAACAGCTTAAGCCGT
R:
CTTTGCCAGAGTCAGCCTGTC
Band lengths:
345-1597
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]