RnoINT0091319 @ rn6
Intron Retention
Gene
ENSRNOG00000013674 | Megf10
Description
multiple EGF-like domains 10 [Source:RGD Symbol;Acc:735084]
Coordinates
chr18:52353327-52354907:+
Coord C1 exon
chr18:52353327-52353563
Coord A exon
chr18:52353564-52354779
Coord C2 exon
chr18:52354780-52354907
Length
1216 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
3' ss Seq
CATTTCCTCTTCTTTTACAGAAA
3' ss Score
11
Exon sequences
Seq C1 exon
CTGGGGTCATCATCGTGGGCAATCTGAACAGCTTAAGCAGGACCAGCACCGCCCTTCCTGCTGACTCCTATCAGATTGGGGCCATCGCGGGCATCGTGGTCCTTCTGCTTGTTGTGCTCTTCCTGCTGGCGCTGTTCGTTATCTACAGACACAAGCAGAAGAGGAAGGAATCGAGCATGCCGGCCGTGACCTACACCCCTGCCATGAGGGTTATCAATGCAGACTATACCATTGCAG
Seq A exon
GTAGGACTGAGGTGCAGGCAGGGCTGCGGTGCCGGGAGGGCTGTGGTGCAGGGAGGCTGCCCTGTGGGAACCCTCCTTGTAAATTTACGTTCTTTGGAATTTGGTGTTTGGCTGTGTGTCTATTGCATCTGATCGGCTCCCCTAACATGACCTCATGGTATATGTGCTTAGAGATATGGGTTACGTCTTAGCAGTAGAAAAATCATCACTTTCATTTTTTCAAAGGCTTTTGAAAAAGGCGCACGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTTTGTTTATGTATGTAGGTACCTGTTGGGACCGGAAGAAGTAGTCAGATTCCCAAGAGAGCTAGAGTTGCAGATGGTTGTGAACTGCTCAATATGGGTGTTTGAGATCTTGGAATTGAACCTGGGTCCTCTGGAAGAGCTTCAAGCATTCTTAACCACTGAGCCATCTTTTCCAGGCGTTCAGCGAGGATTTTTACATGGGGAATCAATGAATGGTTGTTTTCTATTTAGGCAGCTTAATTCATAGATAGGCTCTGCCGAACAAACCAGTTCCAAGTTCTGTCATGTATGAGTGTTGGGGGGAGAAAGAGAGAGAGCACAGTCTCTGCTGTATGTGCTTCAGTCAGCATCCTCCAGCCCTGTGCCTGCACGTGTCAGTAACTGGAGAGAACCAGGGTATACAGGACACAAGCCCGAGGTATTAGCAAGAGCCAAGGCAGTCCCTTATGAAACCTCTTCTGAATCTTCTTGTCACAAGCAGGGTGTGTGTGTAGGGGGCGGGAGACAGCGACAATGCACACATTCAACCATCAAAGGGAGCAAACTCTTACACTGAGGAAACGGAAAATCTTACTCAGTCTTCAGCAATTACCGAAAGACCCTGGCCACATGCTTATAAATATTATGATTCTGAGAGAATATGCAAAATAGGTAATTTACATAAGAGTATGTTTTCGGCGTAGAATTACTGTGTCAGGAAATCAAGTGCTTTTTAAAAGAAGCCGATGGGTATAGATGGTGTAGTTTCTTATGACACTCTGTGTTTACAGGTCCCAAAGCTAAGAGCCGTCTTCTGTGGATGCACTCACAATTTCCAAGGAGGCAGTAGCTGGGCCTGTCCTTCTAGGGGCTTTGAAGAGTGGGCTGGGCTGGAGAGGAAAACATCTTCACTATCGTTTTGATTTAACCATTTCCTCTTCTTTTACAG
Seq C2 exon
AAACCCTGCCTCACAGCAATGGCGGAAATGCCAACAGCCACTACTTCACCAATCCCAGTTATCACACACTTAGCCAGTGTGCCGCGTCCCCTCATGTGAACAACAGGGACAGGATGACCATTGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013674:ENSRNOT00000037901:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.116
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(15.0=7.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCGTGGGCAATCTGAACAGC
R:
GCAATGGTCATCCTGTCCCTG
Band lengths:
350-1566
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]