BtaINT0091656 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000329 | MEGF10
Description
Bos taurus multiple EGF-like-domains 10 (MEGF10), mRNA. [Source:RefSeq mRNA;Acc:NM_001046451]
Coordinates
chr7:27832681-27834307:-
Coord C1 exon
chr7:27834071-27834307
Coord A exon
chr7:27832803-27834070
Coord C2 exon
chr7:27832681-27832802
Length
1268 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
3' ss Seq
ATTTCCTTCTTTGTTGTCAGAAA
3' ss Score
7.61
Exon sequences
Seq C1 exon
CTGGTGTTATCATCGTGGGGAATCTGAACAGCCTCAGCAGGACCAGTACGGCTCTCCCCGCTGACTCCTACCAGATCGGGGCCATCGCGGGCATCATCATTCTCGTCCTAGTTGTTCTGTTCCTCCTGGCGCTGTTCATTATTTATAGACACAAGCAGAAGGGGAAGGAGTCGAGCATGCCAGCGGTTACCTACACCCCTGCCATGAGGGTCATGAATGCAGACTATGCCATTTCAG
Seq A exon
GTGAGACTGGACTCGCTAGAGGCATTTCACTCATGAAAAGCGAGACTTCATCCTGGTTCTTTGAAACCGAGTGTTAGGCTGTGCTGCCCGTTGGTACTGTCCATTAGCCTTGAGCAGCTTTTATGTTTGGAGACGACGCATTGTTAGAGATGTGTTTTATGTGTCAACAGTGGTAAAATCATCACTGTTTTCTCTTATGAGAGATTTAAAATTGGGTTCATTATATGGTTGCTTTAAATGTGGGGAATGTCAACCTAGAAGGAGCTTTGCTGGACGCCTGATCCCAAGCTGTTGCCATATGTGGGGGACAGGGACGGGAGAGGACGAGGTGCCATAACTTCACCAAGGTCATGGAGCAGATCAGGGAAAAAAAAACAGGTCTTCTGTCTTTGGCCTTGGGTTCTTCCCAGGTACCCTCTAGCCTGTCCTGGAGCCCATGTACATTATTAGTCACACAGCAACCAAGTCACATAAACAGTTCAGGGGGATTAGCAAGAACCAAGGCTGTTTTTTTAATAAAACCCCTGAATCTTCCTTTTTGTCACAAGACCCCATAAAAGTAAATTTTTAAGTAATGCATTCAGTCTTCAGAAGATTCATATAAGTGAAGAATTTGCAAACTTAAGGTATTTGTTAATTTGGGGGCAATCAGTGAAGGGTTTCAGGTTTATAAATAAAGTGATTCTGGGGGAATTCATAAAGTAGCTGATTTACTTAGTGGTGTGTTTCTAGTGCAGAGTATTGTCTGGACTTCCCTGGTATTCCAGTGGCTAAGACTCCATGCTCCCAATGCAGGGGGTCTGCCTTCAATCCCTGATCAGGGAACTAGATCCTACATGCCTCAACTAAAACCCAGCACAGTTAGATAGATAGAAATATGGATGGATGGATGGATGGATTGATAGGTAGATAGATTGATTTACAAGTAGATAGAGAAATAGAAAGAATATTGTGTCAAGAGCTCAAGTGTTTAAACACAGAGAAAAGTTACAGTGGAAGAAGCTTCAAAGCAATGCCTTCCCAGCTTGTACAAGCCCCAGAGCAGAGAGCAGTTGTTGGTGGATGCTTTCAGACTTTTCCAGGAGGTGGGCAGAAAGGCAGGCGCCTGCTGCTTCCACAGGCTTCTCTGAGCATGCCGGGCAGGAGGGGATTCTGCCCGCTTGTGATCTGGAATCAGTCCAAACATTTGTTCTTGTTGCTTCACTTCCCTCTTTCCCACACACGTTTAAGGAGCCTGCCATCTTAACCATTTCCTTCTTTGTTGTCAG
Seq C2 exon
AAACCCTTCCTCATGGCAACGGTGGAAATGCTAACAGCCATTACTTCACCAATCCCAGTTACCACACGCTCACTCAGTGTGCCACATCCCCTCACGTCAACAACAGGGACAGGATTGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000329:ENSBTAT00000000434:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.244
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(15.0=7.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGGGAATCTGAACAGCCTCA
R:
TTGGCAATCCTGTCCCTGTTG
Band lengths:
343-1611
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]