MmuINT0097617 @ mm10
Intron Retention
Gene
ENSMUSG00000024593 | Megf10
Description
multiple EGF-like-domains 10 [Source:MGI Symbol;Acc:MGI:2685177]
Coordinates
chr18:57287783-57289410:+
Coord C1 exon
chr18:57287783-57288019
Coord A exon
chr18:57288020-57289282
Coord C2 exon
chr18:57289283-57289410
Length
1263 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGG
5' ss Score
9.46
3' ss Seq
ATTTCCTCTCTTTTTTGCAGAAA
3' ss Score
11.45
Exon sequences
Seq C1 exon
CTGGGGTTATCATCGTGGGCAATCTGAACAGCTTAAGCCGGACCAGCACCGCCCTTCCTGCCGATTCCTATCAGATCGGGGCCATCGCGGGCATCGTGGTCCTCGTTCTTGTTGTGCTCTTCCTGCTGGCGCTGTTCATCATCTACAGACACAAGCAGAAGAGGAAGGAATCAAGCATGCCGGCCGTGACCTACACCCCCGCCATGAGGGTCATCAATGCAGACTATACCATCGCAG
Seq A exon
GTAGGGCTGCTATGCAGGGAGACTGACCTGCAGGGAACCGGTTAGGTTCTTTCGGATCTGGTGTGAGTCCATAGCGTCTGACTGTTTTCATTTTTGTTTTTGTTTTGATGTGACCTCACCTTCTGAAGAGGTTTATGTGCTTCGAGAGTTGGTTTATATCTTAGCAGTAGAAAAATCATCACTTTCATTTTTTTTCAGAGGCTTTTGAAAAGATTTATTTGAGCGAGCGCGCGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTATGTATCTATGTATGTAGTGCCTGGTGGGGCCAAAAGAAGTAGTCAGATCCCCAGGAGAGCCAGAGTTACAGACAGTTGTGAGCTGCCCAACATGGGTGCTCGGGATCTTGGAATTGAATCTGGGTCCTCTGAAAAAGCATCAAGTGATCTTAACCATGGAGCCAACTTTTCCAACCCTTCAATGAGGATTTTTAACTTGGGATCAATGAATGGTTGTTTTCTATTTAGGCAGTTTAATTCATGGATAGTCTCTGCTGAACAGCCAGTCCCAAGTTCTGTCATATATGAGTGTTGAGAGAGAGAGAGCATATATGAGTGTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCACAGTCTCTGTTGTGTGTGCTTCAGTTGGCACCCTCTGGCCCTGTGCCTGCACACGTAAGTAACTAGAAAGAACCAAGGTATAGAGGAGACAAGCCTGAGGCATTAGCAAGAGCCAATGCAGCCCCTCATGAAACCTCTTCTGAATCTTCCTGGTGCAAGTAGTGCGGGGAGGGAGGGGAGAGAGCACCATCAATGCATTCAGTCAACCATCAAAGGGAGAAACACTCAAGTTTTCTCTTACACTGAGGAAACTGAAAATCTTATTCCGTCTTCAGCAACTACTGAAGGACCTTGGCTATATGTTTATAAATATTATGATTCTGGGAGAACATGCAAAATAGGCAATTTACATAAGAGTATGTTTTCAGCACAGAATTATTGTGTCAAGAAATCAAATGATTTTTAAAAGAAGCCCATGGGTGTAGATGCAGTAGTTTCTTAGGACACCATCTTTGTGTTTACAGGCCCCAAAGCTCAGAGCAGTCTTCTGAGAAAGCATTCATAATTTCCAAGGAGGCAGTAGCCCTGCCTGCTCCTTTAGGGGCTTTGAAGAGTGAACTGGGCAGGAGAAGAAAACGTCTGTACAACCATTTTGATTTAACCATTTCCTCTCTTTTTTGCAG
Seq C2 exon
AAACCCTGCCTCACAGCAATGGTGGAAATGCCAACAGCCACTACTTTACCAATCCCAGTTATCACACACTTAGCCAGTGTGCCACATCCCCTCATGTGAACAATAGGGACAGGATGACCATTGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024593:ENSMUST00000075770:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.209
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(15.0=7.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCGTGGGCAATCTGAACAGC
R:
TTGCAATGGTCATCCTGTCCC
Band lengths:
352-1615
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types