DreINT0094985 @ danRer10
Intron Retention
Gene
ENSDARG00000017229 | megf10
Description
multiple EGF-like-domains 10 [Source:ZFIN;Acc:ZDB-GENE-080506-1]
Coordinates
chr10:16191602-16192115:+
Coord C1 exon
chr10:16191602-16191829
Coord A exon
chr10:16191830-16191999
Coord C2 exon
chr10:16192000-16192115
Length
170 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
3' ss Seq
TTTTTCATTTTATTTCCTAGAGT
3' ss Score
9.89
Exon sequences
Seq C1 exon
CTGGAGGGAATATTGCAGAAAGTCCCAATAGTTTGACAAGCGCAGCTCTGCCTATGGATTCATATCAAATTGGCGCAATCACAGGAATCATCATCCTTGTACTTCTTGTGCTCATCCTCCTCCTTCTGTTCATCATTTACCGCAAAAAACAGAAAGGCAAAGAATCCTCGATGCCTTCGGTCACGTATACACCTACTATGAGGGCCAACACTGATTACGCCATTGCAG
Seq A exon
GTCAGTGTCCAAATTGTTGTGATAATACCTCTATTCATTACAATAGCTATACATGTCTCATTATTTTCATAATTAACTGTGTAAGTCAGGGTGACTCCTATGGCAGAACAGGGTAGTTTCAGAAGTAACTGTGTCTGTTTGTGATTTTTTTTTTTCATTTTATTTCCTAG
Seq C2 exon
AGTCACTCCCACAAACAGAGGTCCTTCCCAACAGTAACTATTTCTCCAACCCCAGCTACCACACGCTGACCCAATGCAGCAGCCCACCTCACATCAATAACATACCCTATGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017229:ENSDART00000043936:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.078 A=NA C2=0.051
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(22.7=13.0),PF0200911=Rifin_STEVOR=PD(85.9=71.4),PF061436=Baculo_11_kDa=WD(100=66.2)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGCGCAATCACAGGAATCA
R:
TATTGATGTGAGGTGGGCTGC
Band lengths:
258-428
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]