Special

MmuEX0003603 @ mm9

Exon Skipping

Gene
ENSMUSG00000030972 | Acsm5
Description
acyl-CoA synthetase medium-chain family member 5 [Source:MGI Symbol;Acc:MGI:2444086]
Coordinates
chr7:126671933-126675494:+
Coord C1 exon
chr7:126671933-126672152
Coord A exon
chr7:126673782-126673992
Coord C2 exon
chr7:126675287-126675494
Length
211 bp
Sequences
Splice sites
3' ss Seq
TGGCTGCACTCACTGTCCAGACA
3' ss Score
5.5
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
Exon sequences
Seq C1 exon
GCAGAAGTTGATAGAAGTCATGAGGTTGTGGCTAAGAGGCCTGGCCTGCCAGGCTCTGAGGAGTTCCTGGGGAGTCTGCAGGATTCACACACAGCCACCACCTCCCCCGATCCCTGAGGTGGTAGCTACCTGGGAAGCCATCAGCCTGGGGAGGCAGCCAGTGCCCGAGTACTTCAACTTTGCTCATGATGTGTTGGATGTGTGGAGTCAGCTGGAAAAG
Seq A exon
ACAGGGCACCGGCCCCCAAATCCTGCATTCTGGTGGGTGAACGGCTCAGGAACAGAAGTCAAGTGGACATTTGAGGAGCTGGGGAAGCAGTCGAGGAAGGCAGCCAATGTCTTAGAGGGTGTGTGTGGCCTGCAACCTGGAGACAGAATGATGCTGGTGCTTCCGAGACTCCCAGATTGGTGGCTGATCAGCGTGGCTTGTATGCGAACAG
Seq C2 exon
GTGTGGTCATGATCCCAGGAGTCTCCCAGCTGACAGCGAAGGATCTCAAGTATCGGCTGCAGGCTGCCAGGGCCAAGTCTATCGTCACCAGTGATGCCCTAGCTCCGCAAGTGGATGCCATCAGTGCTGACTGTCCCTCCCTCCAAACCAAGCTTCTGGTGTCTGACACCAGCCGTCCAGGCTGGATCAATTTCCGAGAACTCCTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030972-'1-2,'1-1,2-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.014 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0050123=AMP-binding=PU(0.5=3.0)

							
A:
PF0050123=AMP-binding=FE(16.9=100)

							
C2:
PF0050123=AMP-binding=FE(16.7=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000063416





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr16:20418059-20418269 
HIGH PSI
HsaEX6042600
(ACSM5)
Human
(hg19)
chr16:20429381-20429591 
HIGH PSI
HsaEX6042600
(ACSM5)
Rat
(rn6)
chr1:189247093-189247303 
ALTERNATIVE
RnoEX0006241
(Acsm5)
Cow
(bosTau6)
chr25:18213600-18213810 
HIGH PSI
BtaEX0002257
(ACSM5)
Chicken
(galGal4)
chr14:14914742-14914952 
HIGH PSI
GgaEX1006064
(ACSM3)
Chicken
(galGal3)
chr14:15244477-15244687 
HIGH PSI
GgaEX6013225
([1])
Zebrafish
(danRer10)
chr2:44890849-44891059 
ALTERNATIVE
DreEX0010852
(acsm3)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TCTGCAGGATTCACACACAGC

				
R: 
TTTGGAGGGAGGGACAGTCAG

				
Band lengths: 
293-504

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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