Special

MmuEX0022193 @ mm9

Exon Skipping

Gene
ENSMUSG00000022487 | Gtsf1
Description
gametocyte specific factor 1 [Source:MGI Symbol;Acc:MGI:1921424]
Coordinates
chr15:103251621-103258815:-
Coord C1 exon
chr15:103258771-103258815
Coord A exon
chr15:103255862-103255962
Coord C2 exon
chr15:103251621-103251747
Length
101 bp
Sequences
Splice sites
3' ss Seq
TGTATTTTTCTCTCTTTCAGTCG
3' ss Score
10.92
5' ss Seq
AAGGTAGGG
5' ss Score
8.76
Exon sequences
Seq C1 exon
CACTTGAACAGGACTCCTTTGCCATCAGCATGGAAGACACTTACA
Seq A exon
TCGACTCCCTGGACCCTGAAAAGTTATTACAGTGCCCCTATGATAAAAATCACCAGATCAGGGCCTGCAGGTTTCCTTATCATCTTATCAAGTGCAGAAAG
Seq C2 exon
AATCATCCTGATGTCGCAAACAAATTGGCTACTTGTCCCTTCAATGCTCGGCATCAGGTTCCCCGGGCTGAGATCAGTCATCACATCTCGAGCTGTGATGACAAGAGCTGTATTGAGCAGGATGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000022487_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.252 A=0.000 C2=0.018
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
PF052537=zf-U11-48K=WD(100=79.4)

							
C2:
PF052537=zf-U11-48K=PD(0.1=0.0),PF052537=zf-U11-48K=WD(100=62.8)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000115670





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000023129, ENSMUSP00000114244, ENSMUSP00000114733, ENSMUSP00000115123, ENSMUSP00000116366, ENSMUSP00000119234, ENSMUSP00000120797, ENSMUSP00000122193
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr12:54465067-54465167 
ALTERNATIVE
HsaEX0029063
(GTSF1)
Human
(hg19)
chr12:54858851-54858951 
ALTERNATIVE
HsaEX0029063
(GTSF1)
Rat
(rn6)
chr7:145044301-145044401 
HIGH PSI
RnoEX0040610
(Gtsf1)
Cow
(bosTau6)
chr5:25736462-25736562 
HIGH PSI
BtaEX6020245
(GTSF1)
Chicken
(galGal4)
chr27:1062233-1062324 
HIGH PSI
GgaEX1027847
(GTSF1)
Chicken
(galGal3)
chr27:1016780-1016884 
Zebrafish
(danRer10)
chr23:32175310-32175428 
ALTERNATIVE
DreEX0004939
(GTSF1 (1 of 2))
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ACTTGAACAGGACTCCTTTGCC

				
R: 
CCACATCCTGCTCAATACAGCT

				
Band lengths: 
171-272

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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