MmuEX0026058 @ mm10
Exon Skipping
Gene
ENSMUSG00000015647 | Lama5
Description
laminin, alpha 5 [Source:MGI Symbol;Acc:MGI:105382]
Coordinates
chr2:180197036-180197478:-
Coord C1 exon
chr2:180197373-180197478
Coord A exon
chr2:180197226-180197275
Coord C2 exon
chr2:180197036-180197141
Length
50 bp
Sequences
Splice sites
3' ss Seq
TCCTGCTCTGCTCTCCTCAGGCT
3' ss Score
9.47
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
Exon sequences
Seq C1 exon
ACACAGGCTCCCTGTATGTGCCGGGCTCACGTGGAAGGGCCAAGCTGTGATCGCTGTAAACCTGGGTACTGGGGGCTGAGCGCCAGCAACCCTGAAGGCTGCACAC
Seq A exon
GCTGCAGCTGTGACCCACGAGGCACCCTGGGTGGAGTTACTGAGTGCCAG
Seq C2 exon
GGCAATGGGCAGTGCTTCTGCAAGGCTCACGTGTGTGGCAAGACCTGTGCAGCCTGCAAGGATGGCTTCTTTGGCCTGGATTATGCTGACTACTTTGGCTGCCGTA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015647-'33-33,'33-32,34-33=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0005319=Laminin_EGF=PU(30.0=88.2)
C2:
PF0005319=Laminin_EGF=PD(66.0=91.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCAAGCTGTGATCGCTGTAA
R:
AAAGAAGCCATCCTTGCAGGC
Band lengths:
140-190
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types