Special

MmuEX0028768 @ mm9

Exon Skipping

Gene
ENSMUSG00000041975 | Mettl8
Description
methyltransferase like 8 [Source:MGI Symbol;Acc:MGI:2385142]
Coordinates
chr2:70814131-70820252:-
Coord C1 exon
chr2:70819912-70820252
Coord A exon
chr2:70818434-70818483
Coord C2 exon
chr2:70814131-70814194
Length
50 bp
Sequences
Splice sites
3' ss Seq
TTGTTTATTCTCAATTTTAGGTT
3' ss Score
12.25
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
TTAAGTTTGAAAGTGACGCTAACAAATACTGGGATATATTTTACCAGACCCATAAGAATAAGTTTTTCAAGAATCGTAATTGGCTGTTGAGGGAATTTCCTGAAATCCTTCCTGTTAATCAAAACACTAAAGAGAAAGTGGGAGAATCATCCTGGGATCAAGTCGGAAGCAGCATCTCTAGGACACAAGGAACAGAAACCCATTGTCAAGAGAGTTTTGTCTCGCCAGAACCTGGGAGCAGAGGACGGTCTGCTCCTGACCCCGACTTGGAAGAATACAGCAAAGGACCTGGGAAGACAGAGCCGTTTCCTGGTAGCAATGCCACTTTTCGAATACTAGAG
Seq A exon
GTTGGCTGTGGTGCTGGAAATAGCGTGTTTCCAATCCTGAACACCTTGCA
Seq C2 exon
GAACATTCCAGGATCCTTTCTCTACTGCTGCGACTTTGCCTCTGAAGCTGTGGAACTTGTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000041975_MULTIEX3-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





Show structural model

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.518 A=0.065 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF134891=Methyltransf_23=PU(15.8=26.3)

							
A:
PF082417=Methyltransf_11=FE(17.8=100)

							
C2:
PF082417=Methyltransf_11=FE(23.3=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000107804





     
                   









    
Main Skipping Isoform:
ENSMUSP00000107800





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000035370, ENSMUSP00000068750, ENSMUSP00000088359, ENSMUSP00000097615, ENSMUSP00000107804f4318A, ENSMUSP00000113642, ENSMUSP00000115855, ENSMUSP00000119863
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr2:172193963-172194012 
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr2:25142248-25142297 
ALTERNATIVE
BtaEX0022032
(METTL8)
Chicken
(galGal4)
chr7:17553683-17553732 
HIGH PSI
GgaEX1036002
(METTL8)
Chicken
(galGal3)
chr7:19506024-19506073 
HIGH PSI
GgaEX1036002
(METTL8)
Zebrafish
(danRer10)
chr9:3553091-3553140 
HIGH PSI
DreEX6034825
(mettl8)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CCTGACCCCGACTTGGAAGAA

				
R: 
ACAGCTTCAGAGGCAAAGTCG

				
Band lengths: 
138-188

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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