MmuEX0034183 @ mm10
Exon Skipping
Gene
ENSMUSG00000053965 | Pde5a
Description
phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499]
Coordinates
chr3:122835553-122841048:+
Coord C1 exon
chr3:122835553-122835640
Coord A exon
chr3:122839542-122839642
Coord C2 exon
chr3:122840971-122841048
Length
101 bp
Sequences
Splice sites
3' ss Seq
GTTTTTCTTTTTATATAAAGGGC
3' ss Score
6.11
5' ss Seq
TAAGTAAGT
5' ss Score
8.07
Exon sequences
Seq C1 exon
GAGCGAGCACCCGCTGGCTCAGCTGTACTGTCACTCCATCATGGAACACCATCATTTTGACCAGTGCTTGATGATTCTAAACAGCCCA
Seq A exon
GGCAACCAGATTCTCAGTGGCCTCTCCATTGACGAATATAAGACCACACTGAAAATAATCAAGCAAGCAATTTTAGCCACAGACCTAGCACTGTACATTAA
Seq C2 exon
GAGACGAGGAGAATTTTTTGAACTTATACGAAAAAATCAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053965-'27-23,'27-22,28-23
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.045 C2=0.000
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(12.1=100)
A:
PF0023314=PDEase_I=FE(13.8=100)
C2:
PF0023314=PDEase_I=FE(10.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CAGCTGTACTGTCACTCCATCA
R:
AGAAACAACTCCTTTTGAAGAGGA
Band lengths:
147-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types