Special

MmuEX0035119 @ mm9

Exon Skipping

Gene
ENSMUSG00000030660 | Pik3c2a
Description
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide [Source:MGI Symbol;Acc:MGI:1203729]
Coordinates
chr7:123537715-123562095:-
Coord C1 exon
chr7:123560967-123562095
Coord A exon
chr7:123549374-123549477
Coord C2 exon
chr7:123537715-123537872
Length
104 bp
Sequences
Splice sites
3' ss Seq
AACCACTTTTTTGATTACAGAAA
3' ss Score
5.31
5' ss Seq
GAAGTAAGG
5' ss Score
6.38
Exon sequences
Seq C1 exon
CTGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGAAGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGAAAAAATAGCAAGTACCTCAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAATCTACAGATATCTCCAAAGTCTGAAGACATCAATAAGTTTGATTGGTTAGACTTGGATCCTCTGAGTAAGCCTAAGGTAGACTATGTGGAGGTGTTAGAACATGAAGAAGAGAAGAAGGATCCAGTTTTGCTAGCAGAGGATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCGCCAAGTTGTCACCTAGAAAGAAAGGTGAATGGAAAATCCCTTTCTGGGGCAACTGTAACAAGAAGCCAGTCTTTAATCATTCGGACAGCTCAATTTACAAAAGCCCAGGGCCAAGTATCTCAG
Seq A exon
AAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTACAAGAATTTGAAGTACAGAATGACGAGGTGGCAGCTTTTTGTCAATCCATTATGAA
Seq C2 exon
ATTGAAGACCAAATTTCCATATACTGATCACTGCACAAATCCAGGCTATTTGTTAAGTCCAGTGACAGTGCAAAGAAACATGTGTGGGGAGAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGCTTCAACTACCAGTTACTTTTACATGTGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030660-'6-10,'6-6,8-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.281 A=0.286 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
PF0079413=PI3K_rbd=PU(32.7=63.0)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000126092





     
                   









    
Main Skipping Isoform:
ENSMUSP00000126092f2756A





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000081710
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr11:17155526-17155629 
HIGH PSI
HsaEX0047502
(PIK3C2A)
Human
(hg19)
chr11:17177073-17177176 
HIGH PSI
HsaEX0047502
(PIK3C2A)
Rat
(rn6)
chr1:185252583-185252682 
ALTERNATIVE
RnoEX0065936
(Pik3c2a)
Cow
(bosTau6)
chr15:35881166-35881269 
HIGH PSI
BtaEX0027091
(PIK3C2A)
Chicken
(galGal4)
chr5:11171190-11171290 
HIGH PSI
GgaEX0008669
(PIK3C2A)
Chicken
(galGal3)
chr5:12876863-12876963 
HIGH PSI
GgaEX0008669
(PIK3C2A)
Zebrafish
(danRer10)
chr18:27039800-27039900 
HIGH PSI
DreEX0055517
(pik3c2a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAGATCGCCAAGTTGTCACCT

				
R: 
TGGAGACCTTCACACTGGCAT

				
Band lengths: 
243-347

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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