Special

MmuEX0041611 @ mm9

Exon Skipping

Gene
ENSMUSG00000025207 | Sema4g
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G [Source:MGI Symbol;Acc:MGI:1347047]
Coordinates
chr19:45072722-45073528:+
Coord C1 exon
chr19:45072722-45072944
Coord A exon
chr19:45073161-45073276
Coord C2 exon
chr19:45073368-45073528
Length
116 bp
Sequences
Splice sites
3' ss Seq
TTCTGCCCTTTTCACATCAGCTG
3' ss Score
6.45
5' ss Seq
CAGGTAGTC
5' ss Score
5.3
Exon sequences
Seq C1 exon
TGCATCACAGATTCATTGCGCAGCCGAGGCTACAACTCATCCCAAGACTTGCCATCCCTGGTTCTGGACTTTGTGAAGTTGCATCCCCTGATGGCTCGGCCTGTGGTGCCCACACGTGGGCGGCCCTTGCTGCTGAAGCGCAATGTACGCTACACACATCTCACAGGAACACACGTCAGCACACCTGCTGGACCCACCTATGACCTGCTCTTTCTGGGCACAG
Seq A exon
CTGATGGCTGGATCCACAAGGCCGTAGTCCTGGGCTCTGGGATGCACATAATTGAAGAGATACAAGTGTTCAGGGAGCCCCAGTCTGTGGACAATCTAGTTATCTCACCAATGCAG
Seq C2 exon
CACAGCCTCTACGTGGGGGCTGCAAGTGGAGTCCTCCAGTTTCCTCTGTCCAGCTGCTCACGCTACCAGTCCTGCTATGACTGCATCCTGGCACGGGACCCCTACTGTGGCTGGGACTCCAGCATCCATGCCTGCATGGTGGCCACCACCGTAGCCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025207-'16-19,'16-18,17-19
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.027 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0140314=Sema=FE(17.1=100)

							
A:
PF0140314=Sema=FE(8.8=100)

							
C2:
PF0143720=PSI=PU(69.2=66.7)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000026225





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr10:100980578-100980693 
HIGH PSI
HsaEX0057131
(SEMA4G)
Human
(hg19)
chr10:102740335-102740450 
HIGH PSI
HsaEX0057131
(SEMA4G)
Rat
(rn6)
chr1:264748206-264748321 
HIGH PSI
RnoEX6007538
(Sema4g)
Cow
(bosTau6)
chr26:21686451-21686566 
HIGH PSI
BtaEX0031886
(SEMA4G)
Chicken
(galGal4)
chr6:22629008-22629123 
ALTERNATIVE
GgaEX1051660
(SEMA4G)
Chicken
(galGal3)
chr6:24514157-24514272 
Zebrafish
(danRer10)
chr12:33734033-33734148 
HIGH PSI
DreEX6070271
(sema4gb)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CATCCCAAGACTTGCCATCCC

				
R: 
GAGCAGCTGGACAGAGGAAAC

				
Band lengths: 
245-361

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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