Special

MmuEX0043415 @ mm10

Exon Skipping

Gene
ENSMUSG00000068323 | Slc4a5
Description
solute carrier family 4, sodium bicarbonate cotransporter, member 5 [Source:MGI Symbol;Acc:MGI:2443220]
Coordinates
chr6:83272045-83277571:+
Coord C1 exon
chr6:83272045-83272178
Coord A exon
chr6:83273050-83273321
Coord C2 exon
chr6:83277513-83277571
Length
272 bp
Sequences
Splice sites
3' ss Seq
CACTTCCCTCTGCCACCCAGGGC
3' ss Score
6.05
5' ss Seq
CAGGTGACC
5' ss Score
6.54
Exon sequences
Seq C1 exon
GGAGTAATGGAGAGCTTCCTGGGCACTGCCATGGCTGGCAGCTTGTTCTGCCTCTTCTCGGGGCAGCCTCTTATCATCCTCAGCAGCACAGGACCCATCCTCATCTTTGAGAAGCTCCTCTTTGACTTCAGCAA
Seq A exon
GGCCAATGGCCTGGACTACATGGAGTTCCGCCTCTGGATTGGTCTTCACTCAGCCATCCAGTGCCTTATTCTGGTGGCCACAGATGCCAGCTTTATCATCAAGTATATCACCCGCTTCACCGAAGAGGGCTTCTCCACCCTCATCAGCTTCATCTTCATCTACGACGCCATCAAAAAGATGATTGGTGCCTTCAAATACTATCCCATTAACACGGACTTCAAGCCTGACTTCATCACCACCTACAAATGCGAGTGCGTCGCTCCTGACACAG
Seq C2 exon
TGAACACAACCACGGTCAATGCCTCAGCCCCACTGGCACCAAACACCAACACGTCTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000068323_MULTIEX1-1/7=C1-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0095516=HCO3_cotransp=FE(18.0=100)

							
A:
PF0095516=HCO3_cotransp=FE(17.5=100)

							
C2:
PF0095516=HCO3_cotransp=FE(3.6=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000041007





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000109532, ENSMUSP00000109533
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr2:74247036-74247160 
ALTERNATIVE
HsaEX0059828
(SLC4A5)
Human
(hg19)
chr2:74474163-74474434 
ALTERNATIVE
HsaEX0059828
(SLC4A5)
Rat
(rn6)
chr4:114969475-114969746 
HIGH PSI
RnoEX6014029
(Slc4a5)
Cow
(bosTau6)
chr11:10377321-10377592 
ALTERNATIVE
BtaEX6043064
(SLC4A5)
Chicken
(galGal4)
chr22:2848010-2848281 
HIGH PSI
GgaEX7011581
(SLC4A5)
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
chr5:27473428-27473699 
HIGH PSI
DreEX6011063
(SLC4A5 (1 of 2))
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TAATGGAGAGCTTCCTGGGCA

				
R: 
AGAGACGTGTTGGTGTTTGGT

				
Band lengths: 
188-460

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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