MmuEX0044482 @ mm10
Exon Skipping
Gene
ENSMUSG00000025323 | Sp4
Description
trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595]
Coordinates
chr12:118254410-118300192:-
Coord C1 exon
chr12:118298638-118300192
Coord A exon
chr12:118261727-118261955
Coord C2 exon
chr12:118254410-118254609
Length
229 bp
Sequences
Splice sites
3' ss Seq
TTTTCCGTCTTTTTTTGTAGGTC
3' ss Score
12.65
5' ss Seq
AAGGTAACT
5' ss Score
9.01
Exon sequences
Seq C1 exon
TCAACAAGTACAGATTGTAGGCCAGCCTATCCTGCAGCAAATCCAGATCCAGCAGCCTCAGCAGCAGATTATTCAAGCTATCCCACCACAGTCATTTCAGCTCCAGTCAGGGCAGACAATTCAGACCATCCAGCAGCAGCCTTTGCAGAATGTTCAACTTCAAGCAGTAAACCCGACTCAGGTACTTATCAGGGCTCCAACTTTAACACCTTCAGGGCAAATCAGCTGGCAAACTGTCCAGGTCCAGAATATTCAAAGTCTTTCAAATTTGCAAGTTCAGAATGCTGGGTTATCCCAGCAGTTAACCATCACCCCAGTGTCTTCAAGTGGTGGCACAACTCTTGCTCAGATTGCTCCTGTGGCTGTTGCTGGTGCCCCAATAACTTTGAATACTGCCCAGCTTGCATCAGTGCCTAACCTTCAGACAGTGAGCGTTGCCAACCTGGGTGCTGCGGGTGTTCAAGTTCAGGGAGTTCCAGTAACAATCACTAGTGTTGCAG
Seq A exon
GTCAACAGCAAGGACAGGATGGAGTGAAAGTCCAACAAGCTACTATAGCTCCTGTAACGGTGGCAGTTGGAGGGATTGCTAATGCGACAATAGGTGCTGTTAGTCCTGACCAACTCACACAAGTGCATTTGCAGCAAGGCCAGCAGACCTCTGATGCAGAGGTGCAACCTGGCAAGAGGCTCCGAAGAGTTGCCTGCTCCTGTCCTAACTGTAGGGAAGGAGAAGGAAG
Seq C2 exon
GGGCAGCAGTGAACCAGGAAAGAAGAAGCAGCATGTTTGCCACATTGAAGGGTGTGGGAAAGTATATGGCAAGACCTCTCACCTCCGAGCACATCTCCGATGGCACACTGGAGAAAGACCGTTCATATGCAACTGGATGTTTTGTGGCAAAAGATTCACAAGGAGCGATGAGCTTCAGAGACACAGAAGGACCCACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025323_MULTIEX1-4/4=3-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.522 A=0.260 C2=0.309
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF134651=zf-H2C2_2=WD(100=41.2),PF134651=zf-H2C2_2=PU(41.7=14.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTCTTCAAGTGGTGGCACA
R:
AACGGTCTTTCTCCAGTGTGC
Band lengths:
307-536
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types