Special

MmuEX0046411 @ mm9

Exon Skipping

Gene
ENSMUSG00000029192 | Tbc1d14
Description
TBC1 domain family, member 14 [Source:MGI Symbol;Acc:MGI:1098708]
Coordinates
chr5:36867579-36914686:-
Coord C1 exon
chr5:36913945-36914686
Coord A exon
chr5:36885826-36885946
Coord C2 exon
chr5:36867579-36867697
Length
121 bp
Sequences
Splice sites
3' ss Seq
CTTTTCTTTTCTTTCTATAGGAA
3' ss Score
12.88
5' ss Seq
AAGGTAAGG
5' ss Score
10.51
Exon sequences
Seq C1 exon
AACCCAGAGCCTGTTCCCTGCAGTGTGGTCCATGTGAAGAGAAAGCAGTCTGAGTCAGAGATCGTCCCGGAGCGGGCCTTCCAGAGTGCATGCCCGCTGCCGTCGTGCACACCCTCAGCTCCCACCTGCAGCGAGCGGGAGCAGGTTGTGCGGAAGTCTTCCACATTTCCTAGGACAGGCTATGACTCAGTGAAACTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAAGTGACAATGTCTCTGTCTGCAGTGTGTCTAGTCTTGGCACAGAGCTGTCAACTACGTTGTCGGTCAGCAATGAGGACATCTTGGACCTCATGGTCACGAGCAATTCCAGCGCTATTGTGACCCTGGAGAATGATGATGACCCACAGTTTACTGACGTCACCCTGAGCTCCATCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTGCTGAGACTGAGGAGGGGAGGAAATTGAAACTATTGCACCCATTCAGTCACTTCTTTACAAG
Seq A exon
GAATTTGCTTGCTAGAAAACAAAATGCAAGGCTTGATAGACAGAGAGACTTGGGATGGAAGCTGTTTGGAAAAGTCCCACTCCGAGAGACTGCTCAGAAAGATTCAAAGAAGACCCAGAAG
Seq C2 exon
GAATATGAAGACAAGGCTGGAAGACCGAGCAGGCCACCCTCTCCCAAGCAGAATGTGAGGAAGAACCTGGATTTTGAGCCACTGTCCACCACGGCGCTCATCCTCGAGGACAGACCTGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000029192_MULTIEX1-1/11=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.320 A=0.268 C2=0.712
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0056613=RabGAP-TBC=PU(30.3=23.8)

							
A:
NO

							
C2:
PF085986=Sds3=PU(27.8=62.5)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000116519





     
                   









    
Main Skipping Isoform:
ENSMUSP00000133477





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000031094, ENSMUSP00000084830, ENSMUSP00000098830, ENSMUSP00000115467, ENSMUSP00000116519f2543A, ENSMUSP00000116735, ENSMUSP00000117414, ENSMUSP00000119347, ENSMUSP00000121516, ENSMUSP00000121816
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr4:6967304-6967424 
HIGH PSI
HsaEX0063773
(TBC1D14)
Human
(hg19)
chr4:6969031-6969151 
HIGH PSI
HsaEX0063773
(TBC1D14)
Rat
(rn6)
chr14:79387333-79387453 
Cow
(bosTau6)
chr6:118247881-118248001 
HIGH PSI
BtaEX6032859
(TBC1D14)
Chicken
(galGal4)
chr4:79461633-79461753 
HIGH PSI
GgaEX0022805
(TBC1D14)
Chicken
(galGal3)
chr4:82854113-82854233 
HIGH PSI
GgaEX0022805
(TBC1D14)
Zebrafish
(danRer10)
chr7:39417226-39417352 
HIGH PSI
DreEX0077406
(tbc1d14)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCTGTCAACTACGTTGTCGGT

				
R: 
CGGTCTTCCAGCCTTGTCTTC

				
Band lengths: 
255-376

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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