Special

MmuEX1008782 @ mm10

Exon Skipping

Gene
ENSMUSG00000026825 | Dnm1
Description
dynamin 1 [Source:MGI Symbol;Acc:MGI:107384]
Coordinates
chr2:32308471-32312925:-
Coord C1 exon
chr2:32312684-32312925
Coord A exon
chr2:32312127-32312342
Coord C2 exon
chr2:32308471-32309064
Length
216 bp
Sequences
Splice sites
3' ss Seq
TCTGCTCTTGTCGCCGGTAGATC
3' ss Score
8.33
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
Exon sequences
Seq C1 exon
ACCAAGGAGTTTATCTTCTCTGAGCTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGCGGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACACGACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCCGGACGCAG
Seq A exon
ATCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCGGATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCTGGGGTCCCCAG
Seq C2 exon
CCGATCGGGTCAGGCAAGTCCGTCCCGTCCTGAGAGCCCCAGGCCCCCCTTCGACCTCTAACCAGATCCCTCCTACTCCTCGGAGACCTCCCTTACCAAGCCTGCCTGGACAGCTGTTCTGTGACTTGACAGTGGCTCCCCCGGCCCCAAAGCGTCCTATTCATCATCTGTGACTTAGTCTGTTGTAGTGGTGAGCTGACACATCCAGATGTGATGTTGGTGAAAACTTGTGCCCCTCTGTGGTATTGCTCCTGCGACATTCTATAAATATCTATAAATATACCTATATATACATATATATACATATATATGGCTGACACAGCCTCGCCTGTAGTGTCAGAATCAACCACCATGCTGTCCTTGTGGAGTCTTGTGGTCCAACAAGGGAACGCTGTCATCCTGACAACCCCCCCTCCAGTGTACCACCTCCAGTGAGCCCCCCTGTCCTCCTGGCCTGTGAACAGCCATCCCCTTGGCCATCCCAACCCTGCAGCAAGGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026825-'42-59,'42-49,45-59
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.634 A=0.956 C2=1.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0221213=GED=PD(56.5=64.2)

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000108992





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000077461, ENSMUSP00000088618, ENSMUSP00000108977, ENSMUSP00000108979, ENSMUSP00000115523, ENSMUSP00000116054, ENSMUSP00000118914, ENSMUSP00000122679, ENSMUSP00000143955, ENSMUSP00000144145, ENSMUSP00000144264, ENSMUSP00000144309
          









    
Other Skipping Isoforms:
NA
  



Associated events







Conservation


Human
(hg38)
chr9:128250725-128250940 
Human
(hg19)
chr9:131013004-131013219 
HIGH PSI
HsaEX6063182
(DNM1)
Rat
(rn6)
chr3:11341847-11342062 
HIGH PSI
RnoEX0029033
(Dnm1)
Cow
(bosTau6)
chr11:98873388-98873603 
HIGH PSI
BtaEX6046352
(DNM1)
Chicken
(galGal4)
chr17:5024959-5025177 
HIGH PSI
GgaEX6015059
(DNM1)
Chicken
(galGal3)
chr17:5657558-5657776 
HIGH PSI
GgaEX6015059
(E1BV15_CHICK)
Zebrafish
(danRer10)
chr5:1352814-1353020 
HIGH PSI
DreEX6008890
(dnm1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TATCGGCGACATCAACACGAC

				
R: 
CTGGATGTGTCAGCTCACCAC

				
Band lengths: 
304-520

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 2 annotated functions for this event 


PMID: 9148966
HsaEX6063182
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: mutation analysis. ELM ID: ELMI000178; ELM sequence: PSRPNR; Overlap: FULL


PMID: 21689596
HsaEX6063182
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: competition binding, glutathione s tranferase tag, mutation analysis, pull down. ELM ID: ELMI002614; ELM sequence: VPRITIS; Overlap: PARTIAL_RIGHT


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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