MmuEX6013588 @ mm10
Exon Skipping
Gene
ENSMUSG00000033533 | Acsm1
Description
acyl-CoA synthetase medium-chain family member 1 [Source:MGI Symbol;Acc:MGI:2152200]
Coordinates
chr7:119622498-119652633:+
Coord C1 exon
chr7:119622498-119622689
Coord A exon
chr7:119640575-119640734
Coord C2 exon
chr7:119652554-119652633
Length
160 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTTTTGAGTTAGCAG
3' ss Score
0.54
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
Exon sequences
Seq C1 exon
GACTGCCCTAATATGCAGTGGCTGAAGAGTTTCCAGATCTGCAAAGTCTTACAGGGCTTCTCCCTAAGCCCCACCCAGCTGCACAGACGGTTGTTTTCAAGAGTTGGAGCTCCAAGATGGAATGACCATGATTCACCTGAGGAGTTTAACTTTGCAAGTGATGTCCTGGACTACTGGGCTCAAATGGAGGAG
Seq A exon
CAGAAAATTATTGAAGCTGAAGACATCTGACATCTTGTGGTGCATGTCAGACCCAGGATGGATTCTGGCTACCGTGGGGTGCCTGATCGAGCCATGGACATCAGGATGTACAGTCTTCATCCACCACCTCCCTCAATTCGACCCCAAAGTCATTGTAGAG
Seq C2 exon
GTACTGTTCAAATACCCCATCACTCAGTGCCTTGCTGCCCCAGGCGTGTATCGAATGGTTCTTCAGCAGAAAACCTCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033533_MULTIEX1-4/4=C1-C2
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=PU(0.5=3.3)
A:
PF0050123=AMP-binding=FE(12.8=100)
C2:
PF0050123=AMP-binding=FE(6.3=100)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCCTAATATGCAGTGGCTG
R:
AACCATTCGATACACGCCTGG
Band lengths:
249-409
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types