MmuEX6038558 @ mm9
Exon Skipping
Gene
ENSMUSG00000033624 | Pdpr
Description
pyruvate dehydrogenase phosphatase regulatory subunit [Source:MGI Symbol;Acc:MGI:2442188]
Coordinates
chr8:113653506-113660974:+
Coord C1 exon
chr8:113653506-113653595
Coord A exon
chr8:113656777-113656959
Coord C2 exon
chr8:113658508-113660974
Length
183 bp
Sequences
Splice sites
3' ss Seq
CTTTTCTTCTCTCCATATAGTAC
3' ss Score
9.1
5' ss Seq
AAGGTATTA
5' ss Score
7.04
Exon sequences
Seq C1 exon
GAGATGAGTGTGGGCTATGCAAATGGGATCCGGGTGATGAGTATGACGCACACGGGAGAGCCAGGCTTCATGCTGTATATCCCCATAGAG
Seq A exon
TACGCTCTGCATGTCTACAATGAGGTGATGAGTGTTGGACAGAAATATGGAATCCGGAATGCTGGGTATTATGCCCTCAGAAGTCTCCGAATTGAGAAGTTTTTTGCTTTCTGGGGTCAGGACTTAAACACCCTTACCACGCCTTTGGAATGTGGAGGAGAATCTCGAGTGAAGTTAGAGAAG
Seq C2 exon
GGCATAGACTTCATTGGTCGGGATGCCCTGCTGCAGCAGAAGCAGACCGGGGTATATAAGCGCCTTGCTATGTTCATTCTGGACGACCATGACACAGACCTTGATTTGTGGCCTTGGTGGGGAGAACCCATTTACCGGAACGGAAAGTATGCTGGCAAGACCACCAGTAGTGCCTATAGCTACACACTGGAGCGCCATGTATGCCTGGGTTATGTACACAATTTTTCTGAGGACAGTGGGGAAGAGCAGGTGGTGACAACAGATTTCATCAACCGAGGAGAATATGAAATTGACATTGCAGGACATCGGTTTCAGGCCAAGGCCAAGCTCTACCCTGTCACCTCTTTTTTCACTCACAAGCGTAGAAAGGACGATGTGGAGCTGAGCGACTTTCATGGGAAGTAACGCAAGACACTCCCTTCCCACATTCTTGCCATGAGGAGCATCGTTCTCAGAGCCGAGTTTCCCTCCGTCCTGGTCTCCTCACTGAAGGTACCTTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033624-'19-21,'19-20,20-21=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0157116=GCV_T=FE(13.3=100)
A:
PF0157116=GCV_T=PD(25.2=90.2)
C2:
PF086696=GCV_T_C=WD(100=80.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGTGGGCTATGCAAATGGG
R:
TCTTGCCAGCATACTTTCCGT
Band lengths:
244-427
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: