MmuEX6073864 @ mm10
Exon Skipping
Gene
ENSMUSG00000053965 | Pde5a
Description
phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499]
Coordinates
chr3:122776256-122781634:+
Coord C1 exon
chr3:122776256-122776345
Coord A exon
chr3:122778935-122779072
Coord C2 exon
chr3:122781567-122781634
Length
138 bp
Sequences
Splice sites
3' ss Seq
TGCCACCTTTTCTCGTCCAGGTG
3' ss Score
10.66
5' ss Seq
CCTGTGAGT
5' ss Score
7.21
Exon sequences
Seq C1 exon
GACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTCTTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAG
Seq A exon
GTGTTACTTGACCTTGCCAGCTTAATCTTTGAAGAGCAGCAGTCACTGGAAGTCATTCTGAAGAAGATAGCGGCCACTATCATCTCCTTCATGCAGGTGCAGAAGTGCACCATCTTCATTGTGGATGAAGACTGTCCT
Seq C2 exon
GACTCTTTCTCTCGTGTGTTTCACATGGAGTGTGAGGAAGTAGGAAAACCCTCTGATCCTTTAACGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053965-'10-15,'10-8,13-15=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.217
Domain overlap (PFAM):
C1:
PF0159021=GAF=PD(7.9=40.0)
A:
PF0159021=GAF=PU(19.6=67.4)
C2:
PF0159021=GAF=FE(13.9=100)
Main Skipping Isoform:
ENSMUST00000066728fB14356
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACTTTGCGGCCTACCTGG
R:
TCGTTAAAGGATCAGAGGGTTTTCC
Band lengths:
157-295
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types