RnoEX0064351 @ rn6
Exon Skipping
Gene
ENSRNOG00000014443 | Pde5a
Description
phosphodiesterase 5A [Source:RGD Symbol;Acc:620995]
Coordinates
chr2:226956721-226963787:+
Coord C1 exon
chr2:226956721-226956810
Coord A exon
chr2:226961148-226961285
Coord C2 exon
chr2:226963720-226963787
Length
138 bp
Sequences
Splice sites
3' ss Seq
TGTCACCTTTTCTCGTCCAGGTA
3' ss Score
10.86
5' ss Seq
CCTGTGAGT
5' ss Score
7.21
Exon sequences
Seq C1 exon
GACTTTGCTGCCTATCTGGCATTTTGTGGCATTGTTCTTCACAACGCCCAGCTCTATGAAACGTCACTGCTGGAGAACAAAAGAAATCAG
Seq A exon
GTATTACTTGACCTTGCTAGCTTAATTTTCGAGGAGCAGCAGTCATTGGAAGTAATTCTGAAGAAAATAGCCGCCACTATTATCTCCTTCATGCAGGTGCAGAAGTGCACCATCTTCATCGTGGATGAAGACTGCCCT
Seq C2 exon
GACTCGTTCTCTCGTGTGTTTCAAATGGAGTGGGAGGAAGTAGGGAAATCCTCCGAGCCTTTGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014443-'6-5,'6-4,7-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.087
Domain overlap (PFAM):
C1:
PF0159021=GAF=PD(7.9=40.0)
A:
PF0159021=GAF=PU(19.6=67.4)
C2:
PF0159021=GAF=PD(23.3=43.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATTTTGTGGCATTGTTCTTCAC
R:
CTTGTCAAAGGCTCGGAGGAT
Band lengths:
140-278
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]