MmuEX6095867 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123444835-123450124:-
Coord C1 exon
chr4:123449958-123450124
Coord A exon
chr4:123448144-123448258
Coord C2 exon
chr4:123444835-123444952
Length
115 bp
Sequences
Splice sites
3' ss Seq
TAAAAAATCTTTGTTCACAGAGA
3' ss Score
7.17
5' ss Seq
TTCGTGAGT
5' ss Score
7.9
Exon sequences
Seq C1 exon
GAGCTCACTTTGGCAATGGAAGACCAGAAGGAGAACCTAGATACTCTGGAGCACCTGGTTACTACACTGGGCTCCTGTGGCTTTGCCCTGGACCTATCTCAGCATCAAGACAAGATACAGAACCTTAAGAAGGATTTTACAGAGCTGCAGAAGACAGTTCAAGAAAG
Seq A exon
AGAGAAAGATGCATCCACTTGCCAGGAGCAGTTGGATGAATTCCGGAAACTGATTAGAACCTTCCAGAAATGGCTGAAAGAAACTGAAGGGAATGTTCCTCCTGCAAAGACTTTC
Seq C2 exon
GATCTTATATCTGACTGGGAAAGTAAAGGAGCCCTGCTGGGAGAAATCAACGCCAAGGGCACTGCCTTAGAAAGTCTCATCATGGACATCACAGCTCCTGATTCCCAAGCCAAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'99-100,'99-99,100-100
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.142 A=0.170 C2=0.071
Domain overlap (PFAM):
C1:
NO
A:
PF0043516=Spectrin=PU(37.4=81.1)
C2:
PF0043516=Spectrin=FE(33.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATACTCTGGAGCACCTGGT
R:
TTTGGCTTGGGAATCAGGAGC
Band lengths:
243-358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types