MmuEX6095867 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123122078-123127367:-
Coord C1 exon
chr4:123127201-123127367
Coord A exon
chr4:123125345-123125501
Coord C2 exon
chr4:123122078-123122195
Length
157 bp
Sequences
Splice sites
3' ss Seq
TAAAAAATCTTTGTTCACAGAGA
3' ss Score
7.17
5' ss Seq
AAGGTAGGT
5' ss Score
10.29
Exon sequences
Seq C1 exon
GAGCTCACTTTGGCAATGGAAGACCAGAAGGAGAACCTAGATACTCTGGAGCACCTGGTTACTACACTGGGCTCCTGTGGCTTTGCCCTGGACCTATCTCAGCATCAAGACAAGATACAGAACCTTAAGAAGGATTTTACAGAGCTGCAGAAGACAGTTCAAGAAAG
Seq A exon
AGAGAAAGATGCATCCACTTGCCAGGAGCAGTTGGATGAATTCCGGAAACTGATTAGAACCTTCCAGAAATGGCTGAAAGAAACTGAAGGGAATGTTCCTCCTGCAAAGACTTTCGTGAGTGCTAAGGAATTAGAAAAGCAGATTGAACACTTGAAG
Seq C2 exon
GATCTTATATCTGACTGGGAAAGTAAAGGAGCCCTGCTGGGAGAAATCAACGCCAAGGGCACTGCCTTAGAAAGTCTCATCATGGACATCACAGCTCCTGATTCCCAAGCCAAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'68-67,'68-66,69-67=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.140 A=0.170 C2=0.067
Domain overlap (PFAM):
C1:
PF060097=Laminin_II=PU(26.8=46.4)
A:
PF060097=Laminin_II=FE(53.6=100),PF0043516=Spectrin=PU(37.4=81.1)
C2:
PF060097=Laminin_II=PD(17.5=42.5),PF0043516=Spectrin=FE(33.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATACTCTGGAGCACCTGGT
R:
TTTGGCTTGGGAATCAGGAGC
Band lengths:
243-400
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: