RnoEX0051750 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141130073-141134794:-
Coord C1 exon
chr5:141134628-141134794
Coord A exon
chr5:141132788-141132944
Coord C2 exon
chr5:141130073-141130190
Length
157 bp
Sequences
Splice sites
3' ss Seq
TAAAAAAACTTTGTTCACAGAGA
3' ss Score
5.76
5' ss Seq
AAGGTAGGT
5' ss Score
10.29
Exon sequences
Seq C1 exon
GAACTCACATTGGAAATGGAAGACCAAAAGGAAAACCTAGGTACTCTCGAGCACCTAGTTACTGCGCTGGGCTCTTGTGGCTTTGCACTGGATCTGTCTCAGCACCAAGAAAAGATACAGAACCTTAAGAAGGATTTTACAGAGTTGCAGAAGACAGTTCAAGAAAG
Seq A exon
AGAGAAAGATGCATCAAATTGCCAGGAACAATTGGATGAATTCCGGAAACTGATTAGAACCTTCCAGAAATGGCTGAAAGAAACTGAAGGAAACGTTCCACCTGCAGAGACTTTCGTGAGTGCTAAGGAATTAGAGAAGCAGATTGAACACTTGAAG
Seq C2 exon
GGTCTCCTAGATGACTGGGCAGGTAAAGGAGTGCTGGTGGAGGAGATCAACACCAAGGGCACTGCCTTAGAAAGTCTCATCATGGACATCACAGCGCCTGATTCCCAGGCTAAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'77-86,'77-83,79-86
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.214 A=0.245 C2=0.103
Domain overlap (PFAM):
C1:
NO
A:
PF0043516=Spectrin=PU(37.4=81.1)
C2:
PF0043516=Spectrin=FE(33.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCTAGGTACTCTCGAGCACC
R:
CTGGGAATCAGGCGCTGTGAT
Band lengths:
242-399
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]