DreEX6073715 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35541054-35544363:-
Coord C1 exon
chr19:35544197-35544363
Coord A exon
chr19:35541264-35541420
Coord C2 exon
chr19:35541054-35541165
Length
157 bp
Sequences
Splice sites
3' ss Seq
TCTAAATTTGTCGTTCACAGATT
3' ss Score
9.53
5' ss Seq
AAAGTAGGA
5' ss Score
3.63
Exon sequences
Seq C1 exon
GAGGTGAGGTCAGAAATTACCCAAGAGTCGAGCCAGTTAAAAGCGTTTGTCGAGCTGGGTTCAGATCTGAGTCATTCTGGGGTCCTTGCCAATGTACAAAGCCTTCTGGACACAACCAAAGAGGTTTCTGAGGACTTCGCACGCTTGGAATCTAATGTCAATGAGAG
Seq A exon
ATTTGATTCCATCCAGAACTGTGTTCAGCAGTTGTCCGACTTCCGCTCGCGCTCCGGCTCCCTCCTCAGGTGGCTGCAGACAGTACAAGAGCAACTCCCTGCCAAAGAGCCCAACCTAAGCACAGAAGGCCTGCAGAGAAGAGCACAGCAGCTTAAA
Seq C2 exon
GATCTGCTCACGGAATGGGAGACCCAAGGAAACCAAGTTCAGGAGTTAAACAAGAACAGTTCTCAGCTGGAGAACTTGATTATTAACACCACAGCACCCAAGAACAAGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'59-64,'59-63,60-64=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.071 A=0.264 C2=0.553
Domain overlap (PFAM):
C1:
NO
A:
PF0043516=Spectrin=PU(48.3=81.1)
C2:
PF0043516=Spectrin=FE(41.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCCAAGAGTCGAGCCAGTTA
R:
CTTGTTCTTGGGTGCTGTGGT
Band lengths:
257-414
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]