MmuEX7006494 @ mm10
Exon Skipping
Gene
ENSMUSG00000057969 | Sema3b
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:MGI Symbol;Acc:MGI:107561]
Coordinates
chr9:107602874-107603416:-
Coord C1 exon
chr9:107603297-107603416
Coord A exon
chr9:107603122-107603215
Coord C2 exon
chr9:107602874-107602993
Length
94 bp
Sequences
Splice sites
3' ss Seq
CTCCCCTGCCCTGCCCCCAGGAA
3' ss Score
11.56
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
Exon sequences
Seq C1 exon
ACCGAGTGCATGAACTTCGTGAAGCTGCTGCACACCTACAACCACACCCACTTGCTGGCCTGTGGCACAGGGGCTTTCCACCCAACCTGTGCCTTTGTGGAGGTGGGCCACCGGCTGGAG
Seq A exon
GAACCCATGCTTCAACTGGACCGGAGGAAACTTGAGGACGGCAAGGGGAAGACTCCTTATGACCCAAGGCATCGGGCTGCCTCGGTGCTGGTGG
Seq C2 exon
GGGAAGAACTGTATTCTGGGGTGACAGCAGACCTTATGGGCCGGGACTTTACCATCTTTCGAAGCCTTGGTCAGAATCCGAGTCTCCGAACAGAGCCCCATGATTCCCGCTGGCTCAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057969-'22-22,'22-21,25-22=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.411 C2=0.098
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(33.1=100)
A:
PF0140314=Sema=FE(7.0=100)
C2:
PF0140314=Sema=FE(9.0=100)

Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGAGTGCATGAACTTCGTGA
R:
ATGGTAAAGTCCCGGCCCATA
Band lengths:
174-268
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types