Special

MmuINT0015084 @ mm9

Intron Retention

Gene
ENSMUSG00000031543 | Ank1
Description
ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024]
Coordinates
chr8:24231007-24231446:+
Coord C1 exon
chr8:24231007-24231085
Coord A exon
chr8:24231086-24231371
Coord C2 exon
chr8:24231372-24231446
Length
286 bp
Sequences
Splice sites
5' ss Seq
TGGGTAGGA
5' ss Score
5.12
3' ss Seq
AACCCAATGCATCTCTCCAGGTT
3' ss Score
6.73
Exon sequences
Seq C1 exon
GGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATTCTCAGCGAGTCGCGGCTGG
Seq A exon
GTAGGACCCCCCTTGCCTAGCACCTCGACACGCTTGCTCCTATAAGAACACCATTGTGTCCCCATAAGGCAAATGTGTCAGTAATTTCCTTGTCAAGGACTCTGTCCTGGTGCCAGTTCATCTTATTTTGTCTGCAGCAGGCAAAGCCCTTCCTTTAGGTCTCTACCTGACAGTGATCCGAAGGCTCCTGGGTAACCTGAGCATCCTTCTGTAAGGGCTGCACATCAACCGTATCCATGCTGTGCCCGGCCCAGGCTGCTAACTGCAACCCAATGCATCTCTCCAG
Seq C2 exon
GTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAGAACACCTCGGCCTAAGCTGGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031543-Ank1:NM_031158:35
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.259 A=NA C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NA

							
C2:
PF0053117=Death=PU(19.0=61.5)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000113571





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000072612, ENSMUSP00000081050, ENSMUSP00000081051, ENSMUSP00000081051f2840A, ENSMUSP00000106313, ENSMUSP00000106316, ENSMUSP00000112850, ENSMUSP00000113495, ENSMUSP00000113531, ENSMUSP00000113656, ENSMUSP00000116533, ENSMUSP00000117966, ENSMUSP00000133322
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr8:41688231-41688510 
LOW PSI
HsaINT0008060
(ANK1)
Human
(hg19)
chr8:41545749-41546028 
LOW PSI
HsaINT0008060
(ANK1)
Rat
(rn6)
chr16:73765451-73765726 
LOW PSI
RnoINT0017367
(Ank1)
Cow
(bosTau6)
chr27:36291221-36291377 
Chicken
(galGal4)
chr22:2619832-2620057 
GgaINT0136032
(chAnk1)
Chicken
(galGal3)
chr22:2670101-2670326 
GgaINT0136032
(Q90715_CHICK)
Zebrafish
(danRer10)
chr5:68189427-68189571 
ALTERNATIVE
DreINT0000930
(ANK1 (1 of 2))
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCAGTGGAGCAGAAGACAGGA

				
R: 
CAGCTTAGGCCGAGGTGTTCT

				
Band lengths: 
148-434

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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