MmuINT0045012 @ mm10
Intron Retention
Gene
ENSMUSG00000025479 | Cyp2e1
Description
cytochrome P450, family 2, subfamily e, polypeptide 1 [Source:MGI Symbol;Acc:MGI:88607]
Coordinates
chr7:140763739-140764959:+
Coord C1 exon
chr7:140763739-140764027
Coord A exon
chr7:140764028-140764799
Coord C2 exon
chr7:140764800-140764959
Length
772 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGG
5' ss Score
10.51
3' ss Seq
CTGTGCATTGGTCCCTGTAGTTG
3' ss Score
6.23
Exon sequences
Seq C1 exon
AGATGAGTGGTTATTGGCTGATGAGCCACCCTCCTTCTCAACGACACGTTATAAAATCCTTGTTTCTCCTCAGGAAATTCCTACAAATTTAGAGTGGAGCCCCATCGGCACCATGGCGGTTCTTGGCATCACCGTTGCCTTGCTTGTCTGGATCGCCACCCTCCTCCTCGTATCCATCTGGAAACAGATCTATAGAAGTTGGAACCTGCCCCCAGGACCTTTCCCAATTCCTTTCTTTGGAAACATTTTTCAGCTGGATTTGAAGGATATTCCCAAGTCTTTAACCAAG
Seq A exon
GTAAGGGAAACATTGCTGTATTAAGAAAACGAGTTAGAGATTAGATATAATGTGTATATATTAAACTCAGTGCCAGCTGTTATCCAATGACAGATTACCAAGATACTGAATTAACAGTTCAGCCCATCTTGGGCATGGAACACTCACCGTGAGTAGTGCCATACAGGCTCAGAGGCCCTGTACATTTGAAGTGATTATGAGATACTGATGTTGTTGTTAACTTCCTGTGCCAACTGATACAACAGGTGTATCCTGGCTGGTATCAACCTGTGTCCAAGAACAGACTGAAAAGGAAAATGCTGAGCCAGCTGTGACTCCCCAGAATAACAGCATCTGCCTTTTGCTAATGCTGTGAACATTGCATGGACTAACCAGCCCTCAAAATGCTACCTGGCATGGAATTGATGCTTGGTGTCTGTTAAGGATTGTCTTTGCATGTGGCAGGCTAGCTACAGGCTTCAAGAGCATTGGCTCCAAGTTAGCTTGTACTGCTAGGTAAGGTAGAGCCTTAACTTTAGAGGCAAACAAGAATGTGGTGAGTGTTGGCATTGATTGTGCCTGTTTAGGCACAGAGAAGAGAATTGTGTCTAGATAGAAGGAAGGTCCTCCACAAACCCAGAAGCTTGAGGTGGAAAGAGTATCCCCATATCAGACTAGACACAATGCCCTTTCCCCAAGTCCCTCTAACTCTCTGTGAAGAACAGGAACAATAAGAGGAGATGTGGTCTGTCTAAAGCAGGCTAAGTCCTGTTCTGTGCATTGGTCCCTGTAG
Seq C2 exon
TTGGCAAAGCGCTTCGGGCCAGTGTTCACACTGCACCTGGGTCAGAGGCGCATCGTGGTCCTGCATGGCTACAAGGCTGTCAAGGAGGTGCTACTGAACCACAAGAATGAGTTCTCTGGCCGAGGGGACATTCCTGTGTTCCAGGAGTACAAGAACAAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025479:ENSMUST00000026552:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(25.2=44.1)
A:
NA
C2:
PF0006717=p450=FE(11.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGAGCCACCCTCCTTCTCAA
R:
TTGACAGCCTTGTAGCCATGC
Band lengths:
352-1124
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types