MmuINT0045350 @ mm10
Intron Retention
Gene
ENSMUSG00000028713 | Cyp4b1
Description
cytochrome P450, family 4, subfamily b, polypeptide 1 [Source:MGI Symbol;Acc:MGI:103225]
Coordinates
chr4:115641617-115641987:-
Coord C1 exon
chr4:115641846-115641987
Coord A exon
chr4:115641662-115641845
Coord C2 exon
chr4:115641617-115641661
Length
184 bp
Sequences
Splice sites
5' ss Seq
GGGGTAAGG
5' ss Score
8.05
3' ss Seq
GATGTCCCCTTGTCCTGCAGACC
3' ss Score
10.65
Exon sequences
Seq C1 exon
ATCCAGAAGACAGGAGGCCTGGACAAGGTGGTAACTTGGACCGAACAGTTCCCCTATGCCCACCCACTCTGGCTTGGACAATTTATTGTTTTCCTGAACATCTATGAGCCTGACTATGCTAAAGCTGTATACAGCCGAGGGG
Seq A exon
GTAAGGTGCCCTGGAGAGAGTGTGGGGTTTCCTGGGAAAAATGGCCCCAGGGATGAAGTTGGGAGAGGTCCGGACTTGTGTCTAGACTCTGGGAAAAGAATGACCACATCCCTACCTAATTTATCACTATGCCTCATTTGAGAATGTCCTATTTCTCCTTGATTGATGTCCCCTTGTCCTGCAG
Seq C2 exon
ACCCGAAGGCTGCATATGTGTATGACTTCTTCCTCCAGTGGATCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028713:ENSMUST00000102707:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.3=100)
A:
NA
C2:
PF0006717=p450=FE(18.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCAGAAGACAGGAGGCCT
R:
CGATCCACTGGAGGAAGAAGT
Band lengths:
187-371
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types