MmuINT0065120 @ mm9
Intron Retention
Gene
ENSMUSG00000036053 | Fmnl2
Description
formin-like 2 [Source:MGI Symbol;Acc:MGI:1918659]
Coordinates
chr2:52985047-52986010:+
Coord C1 exon
chr2:52985047-52985145
Coord A exon
chr2:52985146-52985886
Coord C2 exon
chr2:52985887-52986010
Length
741 bp
Sequences
Splice sites
5' ss Seq
AAGGTAGGA
5' ss Score
9.45
3' ss Seq
AGTCTCTGCTTTTTTTTTAGTCT
3' ss Score
7.29
Exon sequences
Seq C1 exon
CAAGCAGAAGAGGAAAATGAGCTGAGGAAAAAGCAGGAACAAGCTCTTATGGAAAAACTGCTAGAGCAAGAAGCCCTGATGGAGCAGCAGGATGCAAAG
Seq A exon
GTAGGAAGCCTCTGCTTCTGCAACTGGCCTGAGTGAAGCTCAAGTAGAAAGCAGCGTGAGCCAACTGTGCAAGGTCCTGACCACTGCCTCCTGGTGGCACATGGGAGATAGAGTCACTACTGTGTTTAACAACTTCTAGAATGAATGGGAGTGCTTGCGAAGCCTCGCTAAGTTTACAGAAGTAAGAATTTGGGTGTATATTCAGAAAAATCCATATAAGATTATCTGATGACCAGGCCTTGACCCAGGAAAGGCTCCATGGTATCCAAGGGTACTGACCTTCTCTGATAGTCTTTGCTAGGAGGGTTGGAAGAAAGCATTTCCTGAAGCATGATGCATTATTGATACCCAGCACCCATATAAAAATGCTGGAGGCTTATAATTCAAAGCCATAAAGACAGATGGAGTTGACTATATTATTTATAGTAAAACCAGACTATATTACTTAGCTCCAGGTCAATGAACAGACTCTTCTTACAAAGCAAGGTAGGTATTACTCTTCCACACACAGATACATACACACACTCTTTACATTGCTGCCCGACTGGGTTTTGTGTGCTCATAGGTGAGAGCTTCTGTTGCCTTTCCAGAAGTTTCCTGAAATGTTGGCATGTGAACAGCATGTTGGGATGGTTGTGTCTGCAGTGTTGGCTGTGACTGTTCAGTAAAGCTCGGGCACACTTGGAGGGTCTTTAAAGTCCAGATGGGTACTGGTGTCATCAGTCTCTGCTTTTTTTTTAG
Seq C2 exon
TCTCCTTCCCATAAATCAAAGAGGCAGCAGCAAGAGTTAATTGCGGAATTAAGAAGGCGACAAGTTAAAGATAACAGACATGTATACGAGGGAAAAGATGGCGCCATTGAAGACATTATCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000036053-Fmnl2:NM_172409:24
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.687 A=NA C2=0.569
Domain overlap (PFAM):
C1:
PF0028516=Citrate_synt=FE(20.6=100),PF145131=DAG_kinase_N=FE(21.9=100)
A:
NA
C2:
PF0028516=Citrate_synt=PD(25.8=95.2),PF145131=DAG_kinase_N=PD(20.5=71.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGCAGAAGAGGAAAATGAGCTG
R:
TGTGATAATGTCTTCAATGGCGC
Band lengths:
222-963
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: