MmuINT0094300 @ mm9
Intron Retention
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123063792-123064674:-
Coord C1 exon
chr4:123064471-123064674
Coord A exon
chr4:123063801-123064470
Coord C2 exon
chr4:123063792-123063800
Length
670 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
GACTGCGCCCACCATTGCAGATC
3' ss Score
8.21
Exon sequences
Seq C1 exon
CAACTAAGGGAATCCATTGCTGAACACAAACCTCATATTGATAAGATACTAAAAATAGGTCCACAACTAAAGGAGCTAAACCCTGAGGAAGGGAAAATGGTTGAAGAAAAATACCAGAAAGCAGAAAACATGTATGCCCAAATAAAAGATGAGGTGCGCCAGCGTGCCCTGGCTCTGGATGAGGCTGTGTCCCAGTCTGCTCAG
Seq A exon
GTAAGTGTGACTGTGTCGGGACACTCAACTGTGCACTAGCCTAAGCAGAGGTTCTCTGTTGATGATCCATCATCTGTAGAGCAGAAAACTGTCTGCTTGCATTATTCCCAGTATCGGTGCACTGACGCTGGGTGAGAGGTGAAGTACAGCCTGCCTGGGATGTGTAGTTGTTTATTGATGGTTTCTCTGAGAAATTCATGCTACGATGACTCTTTAGCATCTTAATTAATTTGAGGATAAATTAATTTTGTAGATCCCAAACCAGTAATTACCTTAACAAAAGACAGGTTCCAACTGGGCCTGTTAATGGTAAGATTTAGAATGGTAGCACATTAACGTGAGTAGCCAACTTCAAGTTTAGAAAAAAATATGCATCCAAAGGGAGAAGTACACCAAAGAGAAGGGAATCCCCCAGTGGCAGACCTTACAGCACGGAACTGCACCCTTCTCAACTAATACATTGCTGTTGGTGGCACGTGTGTCGTGTCTGGGTGTTTTACTCCTGTGTTGAGTCAGTATTCTTGGTCAATACTTGTACTTACTCGAGTTTCCCACTCCCGTCCCATCTCACCCCATTGTGGAATGTGTTCCTTTGTGTTGTTGCCGCCTGCTCCTGTCTTCCTCACACCGCCTCATCCCATTGTTGTGTTGACTGCGCCCACCATTGCAG
Seq C2 exon
ATCACAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-Macf1:NM_009600:64
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.279 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(56.6=88.2),PF060097=Laminin_II=PU(52.9=80.9),PF0043516=Spectrin=PU(0.9=1.5)
A:
NA
C2:
PF060097=Laminin_II=FE(1.9=100),PF0043516=Spectrin=FE(1.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: