Special

MmuINT0103467 @ mm9

Intron Retention

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66906639-66907088:+
Coord C1 exon
chr11:66906639-66906815
Coord A exon
chr11:66906816-66906942
Coord C2 exon
chr11:66906943-66907088
Length
127 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGC
5' ss Score
4.72
3' ss Seq
GGAAATGATGCCTCTTGCAGCTG
3' ss Score
5.03
Exon sequences
Seq C1 exon
GTCAAAAACCTTACCGAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCCACCAGCAGACCCTGGACGACCTCCAGGCTGAAGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAAGCTGGAGCAGCAAGTGGATGAT
Seq A exon
GTGAGCACTGGGCATCAGTCTCAGGAGTCTTAAGGCTTCAGGGCGGAAGAACCCTGGGCAGGGGCGGGGCTGGGGTTATCCAATCAGAAATCATTTAATCCTAAAGCGGAAATGATGCCTCTTGCAG
Seq C2 exon
CTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAAAGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACAAGCAACAGCTAGACGAGAGGCTCAAGAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-Myh3:NM_001099635:24
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.424 A=NA C2=0.041
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=FE(29.7=100),PF0157614=Myosin_tail_1=FE(43.3=100),PF0003816=Filament=PU(6.6=30.5)

							
A:
NA

							
C2:
PF0157614=Myosin_tail_1=PD(0.1=0.0),PF0157614=Myosin_tail_1=PD(25.4=69.4),PF0003816=Filament=FE(17.6=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000007301





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:10639190-10639297 
ALTERNATIVE
HsaINT0107735
(MYH3)
Human
(hg19)
chr17:10542507-10542614 
LOW PSI
HsaINT0107735
(MYH3)
Rat
(rn6)
chr10:53636736-53636862 
LOW PSI
RnoINT0096480
(Myh3)
Cow
(bosTau6)
chr19:30237812-30237918 
LOW PSI
BtaINT0097446
(MYHC-EMBRYONIC)
Chicken
(galGal4)
chr18:545424-545508 
Chicken
(galGal3)
chr18:599099-599178 
GgaINT0127425
([1])
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCTAACACGGGAGAAGAAGGC

				
R: 
GCTGTTGCTTGTCGTTCTCCA

				
Band lengths: 
254-381

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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