MmuINT0103474 @ mm10
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67096301-67097058:+
Coord C1 exon
chr11:67096301-67096497
Coord A exon
chr11:67096498-67096874
Coord C2 exon
chr11:67096875-67097058
Length
377 bp
Sequences
Splice sites
5' ss Seq
CAAGTATGT
5' ss Score
7.44
3' ss Seq
TTCTTCTCGTTTTCTTGAAGAAA
3' ss Score
6.64
Exon sequences
Seq C1 exon
GCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTACGAGGAGGAGCAGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAGTATGAAACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAA
Seq A exon
GTATGTGTCTCTAATTAGAACGCTTAAGGGATCTCATAGCTTAGCACACTGGCTCTAGAGTGACTCAGGGATGAATCTAAAGAGTGAATTTGATTTGTTTCTCAAGATTTCCCCATCAAGGAGTCTTTAAATGTAACACTGGCTGAAATGTAAAATGTAACCGAACGAATTCAGTTCATTCAGACACCAGCTTTGATCCTGGTTAGACCGGAGAAAGGCCATTGATCACAGACTCTGAGCAGATGGGGGCATTCTCTTAAGGTTTACGAGCAGAGGTGTGTGAGCCACGCGGTTGGTATCTCCAGCTTGGTGAGCAGTGATGCTCGTGGAGAAGATTGAAACTTAATGACATCAGCTTTCTTCTCGTTTTCTTGAAG
Seq C2 exon
AAAAAAGCTTGCGCAGCGCCTTCAGGATTCGGAGGAGCAGGTAGAGGCTGTAAATGCCAAATGTGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAGGGGAGGTGGAGGACCTGATGGTGGATGTGGAAAGAGCCAACTCCCTGGCTGCCGCTCTGGACAAGAAGCAGAGGAACTTTGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:30
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.567 A=NA C2=0.242
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(19.9=100),PF059116=DUF869=PD(27.3=45.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.1=100),PF045827=Reo_sigmaC=PU(39.6=95.2),PF152721=BBP1_C=PU(20.0=41.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTCCTCCCGCCATGACT
R:
CCTCTGCTTCTTGTCCAGAGC
Band lengths:
342-719
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types