MmuINT0103509 @ mm9
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67066656-67067628:+
Coord C1 exon
chr11:67066656-67067045
Coord A exon
chr11:67067046-67067501
Coord C2 exon
chr11:67067502-67067628
Length
456 bp
Sequences
Splice sites
5' ss Seq
AAGGTCTTT
5' ss Score
2.08
3' ss Seq
TCCTTTACTTCCCATCACAGGGG
3' ss Score
8.74
Exon sequences
Seq C1 exon
GCCCGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCTAAAGCAGAGAAGCAGCGCTCTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCAGATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTGCAGCATGAAGCCACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGATCTCGCTAGTAACATGGAGACAGTCTCCAAAGCCAAG
Seq A exon
GTCTTTCCTAACTTCCTAAATTATCTGAAATACATGAAAGTGATTATATACTCTGTGGGACTCTAGCTAGCCACACTATACATCACCCAAGATCTCTGATTGACATCACTGTATTAAATGGAACCTCTCAAGGTTCCACATCTTATTTTTCAAAATCACCTTAAACATTGTTGCTATAGCTACGGCAATAGTAATGCAGTGGCAAGTAAAAGTCCCCTCTATATGATGTGGGCCATAAATGATGTTCATGTTTCCCCAAAGAAAGGACTCTAGTTTTGATGATTACATATTCACTCAGTCAGTCACATATGCTCCTCCACTGCTGTGATCCCAACTGCTCCCAAGCACTGTGCAAGGCTTGGCAGCAACATCAGTAACAGCCTGTGTCTGCCATACATAGTAACAGATCTCTAAATCTCAGCATCATCTTGTCCCGTCCTTTACTTCCCATCACAG
Seq C2 exon
GGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTGAGTGAAGTGAAGACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAACTGTCAACCCAGAAGGCACGCTTGCACACAGAGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-Myh4:NM_010855:26
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.923 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF087025=Fib_alpha=PD(14.7=13.1),PF0157614=Myosin_tail_1=FE(15.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.9=100),PF059116=DUF869=PU(30.3=62.8),PF0003816=Filament=PU(4.2=30.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGAGGAGATCAGCGAGAG
R:
TCACTCAGTTGGTCCTCCAGG
Band lengths:
348-804
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: