MmuINT0104456 @ mm10
Intron Retention
Gene
ENSMUSG00000025885 | Myo5b
Description
myosin VB [Source:MGI Symbol;Acc:MGI:106598]
Coordinates
chr18:74740474-74742215:+
Coord C1 exon
chr18:74740474-74740625
Coord A exon
chr18:74740626-74742125
Coord C2 exon
chr18:74742126-74742215
Length
1500 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGT
5' ss Score
11
3' ss Seq
CTGTGCTTTTCTTTTCACAGAAG
3' ss Score
10.16
Exon sequences
Seq C1 exon
ACCTGAAGCCTCAGATGCTGTCGGGCACCGTGCCCTGTCTGCCTGCATACATACTCTATATGTGCATCAGGCACGCGGATTACACCAACGATGACCTCAAGGTGCACTCGTTGCTGAGCTCCACCATCAACGGCATTAAGAAAGTCCTCAAG
Seq A exon
GTAAGTAAGCAACCCAGTTCCCAGCAGCCCTAGCAAAGCTGGCACCTCCTTTGCGTGTCTGTCTGTATCTGTGTTCTGCCAGTTATCTGGGATGCCTGTGGGTTCTCTGAGCAGGGTCAGGTCTGCAAATGTCAGGAAGCACAGAACTCCATGTGGGCCTGCAGCAACCTCCCTGCTGAGCCTGGGTTTCCGGGAAAGCCTGTCTGCCCGCTAAGATAGATGCTATCGAGGTGTGCTACCTCTCTCACCATTCCAGGGGGCTGAGGCACACGGTCCTGGCTGGTGGCACAGCAGAGTTGCTGCCGCCGCCATTCATAGGTTTACAAGGTGAGGCAGACTGAGCCAGAACAGGGCTGCTCATAGTGGCGGAGGTAAAACCGCTCACGAGTGCTACCTTCAGGCACGAGATTCTTATGGGATGTATGTAGTAGCAGCTGCTTGTGTTGTTGGCTAGCCTGTTTGGGGGCGAGGCACTTTTACAAAGGAGCTGGAGTCAAGTAGTAGGAGGAGGGCTTTAGAGGGAGATTAGACCTCAGTGACAGTCTGATTCAGATTCTCCCTGACACTGCTGCTCCCAGGAGGCAAGCCACCCCCAAGAGCCCTGAAGCCAGGCCATGTCTGGTCCCTGAGCCTATGATTCCTGATTTGAGAACAAGCTAGTCTTAAGCTTCTGGCTGCAAGCCCTTGGCAGCTGCCCCACCTTCCCTGACTTGCTACATACAATCGGTATCTGCTAATTGCTATGAAGTGTATCATTCACTTGTACAGGCTGATCGCTCTCTAATTGGTGTCTCTGGCCTTGACCTCTTTTACAAGCTCCAGCTGGAGTCTAAGGCAGCCTGGGTCTACAAATGTCCCTTCCTTAGGGAGGCCTCCGTAGCTCTGAGCTAGATTAGCCTGTTCTGGCCCATAGCCTCTGTGGTCAGCCTTGTTTTATCATCCTGGCTCATAGTCATTGCTATTTAAATAATGCTAGATAAATAAATCTACAGAATGGGCTCTGAGAAGTCAGTGGAAGCGATGCAGAAACTGGATGGATCAAGTTGTATATGGTTTGCTAAGCTGCCTTGGCCACTTAGTTTATTGCCACTGACCTGAGCATCAATGGGACACCAGCCTTGCATAGTGCAGAATCAGACTTGGCCAAGTCCAAATTTCAGATCCAACTGGAGCCTGCTGCTGCTGGACCCAAGCATCCTTTGCACCGCTCAGCTTCACCGTCTTCACTCATGGGAGTCAGAGAGATCTTCCCATGTGCAGGGCCATACATCACTCATCAGGAGACTGAGGAGTGACTCTGTCTGTGCCATCTCAGGGAGTGGAGTAATAGCAATAACCGCTCAGCAAAGATTAGCTCTGCTGGGACTGGAAAGCTGACTGGGTACATTGGGCTGCCCAGGAATGCTGGGTAACACAGGAAACGCAGGAAGAGAAAGGCACTGGGGACATATGTCAGCCAATGGGAGGCCCTCAGCCAGTGCTGTGCTTTTCTTTTCACAG
Seq C2 exon
AAGCACAATGATGACTTTGAGATGACGTCATTCTGGCTATCCAACACCTGCCGCTTCCTTCACTGTCTGAAGCAATACAGTGGTGATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025885:ENSMUST00000074157:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGCCTCAGATGCTGTCGG
R:
CTCATCACCACTGTATTGCTTCAGA
Band lengths:
238-1738
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types