MmuINT0127106 @ mm10
Intron Retention
Gene
ENSMUSG00000061136 | Prpf40a
Description
pre-mRNA processing factor 40A [Source:MGI Symbol;Acc:MGI:1860512]
Coordinates
chr2:53151679-53152554:-
Coord C1 exon
chr2:53152446-53152554
Coord A exon
chr2:53151830-53152445
Coord C2 exon
chr2:53151679-53151829
Length
616 bp
Sequences
Splice sites
5' ss Seq
AAGGTACTT
5' ss Score
8.4
3' ss Seq
TATTCAAACATTCATTGTAGGAA
3' ss Score
7.04
Exon sequences
Seq C1 exon
AAAAGCTGAGCAAATGTTTGGGGAGATGGAAGTTTGGAATGCAATTTCAGAGCGTGATCGTCTAGAAATCTATGAAGATGTTTTGTTCTTCCTTTCAAAGAAAGAAAAG
Seq A exon
GTACTTTTAAAATTATCAGTATTTCAACATTCTGAAGAATATTTTACTTTAAATGATATGACAATTTCTTTACTTAATTTGTATAGTGTATGTTATATATCATATTTATTCATTGGAAGCTATGATAAAGATAAAATGAGTTAACTCAATCTTTTTTCCCTCTTTTCAAAGATAGTAATTATTGTCTAAATCATAGTGAGTACTGATTTATCGGAATATTTATCACTGTTAAATTGTTTTGCCTCCATTCTTAAAGGAAACATGGATGATAGTTCAAATATTACTAAAACTTTAAACATAGTTGAATGACATTGAAGACTTTGGTATTTTACATGCAATGACTGCTTTCAGTAGCCCTCATTATAAAATGTATCTGTACTGCATACTGGTAAGGTTATGGAATAAGGACACAAAAACAAATAGTTTTTGGGCTCTGACTTCAAAGACAGATGGAAAAACTAGAGGTAAGACTCTGTCACAAACAACACTAACAAAAGGTGGTGTTACTAAAGGCTTATGTATTATATTTGAAATATCAGAATATAAAGGAATCTTAAGTAATTGCAATTCAGATGCCTTGTTTGAATGTTAAAAGGTATTCAAACATTCATTGTAG
Seq C2 exon
GAACAAGCAAAGCAGCTACGAAAGAGAAATTGGGAAGCCTTAAAAAACATACTTGACAATATGGCTAACGTTACATACTCTACCACTTGGTCTGAAGCCCAGCAGTATCTGATGGATAATCCAACGTTCGCAGAGGATGAAGAGTTACAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000061136:ENSMUST00000076313:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=NA C2=0.196
Domain overlap (PFAM):
C1:
PF0184614=FF=PD(54.0=73.0)
A:
NA
C2:
PF0184614=FF=PU(75.0=82.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGCTGAGCAAATGTTTGGGG
R:
AACTCTTCATCCTCTGCGAACG
Band lengths:
254-870
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types