MmuINT0127113 @ mm10
Intron Retention
Gene
ENSMUSG00000061136 | Prpf40a
Description
pre-mRNA processing factor 40A [Source:MGI Symbol;Acc:MGI:1860512]
Coordinates
chr2:53144784-53145370:-
Coord C1 exon
chr2:53145233-53145370
Coord A exon
chr2:53144880-53145232
Coord C2 exon
chr2:53144784-53144879
Length
353 bp
Sequences
Splice sites
5' ss Seq
GATGTATGT
5' ss Score
6.31
3' ss Seq
TCCATATCGTTGCTTTCCAGATC
3' ss Score
9.13
Exon sequences
Seq C1 exon
TTACTAGAAAAGGCAGAAGCCCGTGAACGTGAGAGAGAAAAAGAAGAGGCTCGGAAAATGAAACGGAAAGAGTCTGCCTTTAAGAGTATGTTGAAACAGGCTACCCCTCCAATAGAACTGGATGCTGTCTGGGAAGAT
Seq A exon
GTATGTATACTTGTGCTTCAAAGTTGAAAATAACACTAATTTGTTTCTAGGGGACACAGATAAGCTAAACTATTCACAAAATATTCTATATATGTCAAAAGCTAGTTGTGACTTGAATTTCGGAATAGTTTGTCTAAATGGTTTGAATACAGCACCAGCAGGGGACAGGGCTTAGATCTATGTACTTGATTCTGCTTCCCAGCATGCACTGCCACACCCTGCTAGAACTATTGAAGGAGAAAAGAGTCCACTTTTAAGTGAATCTCAGGTTTCTCCATGGGTCATACTGCTTTTAGATATGATGATAAGATTTTCTTATCTTCATATATTTATTCCATATCGTTGCTTTCCAG
Seq C2 exon
ATCCGGGAAAGATTTGTAAAGGAACCAGCGTTTGAGGATATAACTCTAGAATCTGAAAGAAAAAGAATATTTAAAGATTTCATGCATGTGCTTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000061136:ENSMUST00000076313:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.543 A=NA C2=0.188
Domain overlap (PFAM):
C1:
PF0184614=FF=PU(46.2=52.2)
A:
NA
C2:
PF0184614=FF=PD(50.0=81.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGAAGCCCGTGAACGTG
R:
CTCAAGCACATGCATGAAATCT
Band lengths:
222-575
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types