MmuINT0140043 @ mm9
Intron Retention
Gene
ENSMUSG00000038677 | Scube3
Description
signal peptide, CUB domain, EGF-like 3 [Source:MGI Symbol;Acc:MGI:3045253]
Coordinates
chr17:28301619-28302166:+
Coord C1 exon
chr17:28301619-28301735
Coord A exon
chr17:28301736-28302007
Coord C2 exon
chr17:28302008-28302166
Length
272 bp
Sequences
Splice sites
5' ss Seq
CAGGTTTGG
5' ss Score
5.63
3' ss Seq
GACACGGCCTCTGTTTCCAGGTA
3' ss Score
9.37
Exon sequences
Seq C1 exon
AGGCTGCAGTGCTGCCAGTCAAGCAGAGAGCCTCCTTCAAGATCAAGGATGCCAAATGTCGCCTGCATCTGCGAAACAAAGGCAAAGCCGAGGAGGCCAGCAGGATCCTGGGGCCAG
Seq A exon
GTTTGGACCTCCCTCCCTGGCCCCACCAGGCTCTCCCCACCTCTCAGCCCCTCTGCCTAGTTCTGTCATGGATTCACCTGCACCCTGGGCTGTGGATAGAATGGGGAAGCTGTTGTAGGCTAAGGCCCTGGAAGTCCCCGTCATTCCCTCCTACCGAGAAGAGGGTTGGGGGTAGGGATGTCTGGGGCGGATGGAGAAATAGGAGAGTCAGAAGAGAGAACTTGTCTTCAGTGGAAGCTACAGTTCGAGGTTGACACGGCCTCTGTTTCCAG
Seq C2 exon
GTAGTGTCCCCTGCTCTGACTGCCTGGTCACTTTCATCCACCTTAAGTGTGACTCTTCTCGGAAGGGCAAGGGCAGGCGGGCCCGGACCCCCCCAGGCAAGGAGGTTACCCGGCTCACCCTGGAATTGGAAGCTGAGGTCAGAGCAGAAGAAACGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000038677-Scube3:NM_001004366:13
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.050 A=NA C2=0.370
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTGCTGCCAGTCAAGCA
R:
TGTCGTTTCTTCTGCTCTGACC
Band lengths:
269-541
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: