Special

MmuINT0179370 @ mm9

Intron Retention

Gene
ENSMUSG00000043059 | Zfp513
Description
zinc finger protein 513 [Source:MGI Symbol;Acc:MGI:2141255]
Coordinates
chr5:31503956-31504676:-
Coord C1 exon
chr5:31504360-31504676
Coord A exon
chr5:31504112-31504359
Coord C2 exon
chr5:31503956-31504111
Length
248 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGG
5' ss Score
6.23
3' ss Seq
AAGCACCCTCCCCTCTTTAGTGG
3' ss Score
4.99
Exon sequences
Seq C1 exon
GGTGGCGGAAGCGGAAGCGGGTACGGAGGTACCAGCTGGTCTTCGGAGGGGGGTAGGGGGCTCCATGAATGGAAGCGGCGGCGGCGGCGGGAGCGACCTGAGCTGGATTCCGGGGCCGGGGCCAGGGGCTGCCCAGGGCCCGCACCGGTGTATGGGGGCGGTTCGTGGATCCTAAGAGCAAGGACCGACGGCAGGGCCGAACTGGGAGGGGCGGGGCCGGGAGGCCTCGGGCCGGAGGCGCGTCGGGCTGGAGCCGGTCACGATGCCCCGAAGGAAGCAAAGCCATCCACAGCCCGTGAAATGCGAGGGGGTCAAAG
Seq A exon
GTCAGGGGTCAGGGGCCTTGAGCCGGGAGGAACAGGGGTGGGGTCAGTAGAGTGGGCTCAGGTCAGGGTGGAGGGGGACTCCTGAGGGTTAGGGGCGGATGATCTGGGATCTTCGCTCCTTACCAGAGTATTAAAGGAACCTGAGGGTCATCGAGTACGGGAAGTGCAGTTCACAACAGCTGGCTCCTTGGTTCGGATTATGGGTACTGCTTGGGAGGGAGATTCCACAAGCACCCTCCCCTCTTTAG
Seq C2 exon
TGGATACTGAAGATTCCTTCGACGAAGGTCCTGGGGCCCTGGTGTTGGAGAGCGATTTGCTACTAGGCCAAGATCTGGAGTTTGAAGAGGAAGAGGAAGAGGATGAAGGTGACGGCCACAACGACCAGCTCATGGGCTTTGAGAGAGACTCTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000043059-Zfp513:NM_175311:1
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion (1st CDS intron)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=1.000 A=NA C2=1.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NA

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000031562





     
                   









    
Main Skipping Isoform:
ENSMUSP00000110238





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000134904
          









    
Other Skipping Isoforms:
ENSMUSP00000085307
  



Associated events







Conservation


Human
(hg38)
chr2:27380249-27380471 
ALTERNATIVE
HsaINT0187485
(ZNF513)
Human
(hg19)
chr2:27603116-27603338 
ALTERNATIVE
HsaINT0187485
(ZNF513)
Rat
(rn6)
chr6:26537972-26538219 
ALTERNATIVE
RnoINT0168333
(Zfp513)
Cow
(bosTau6)
chr11:72273874-72274115 
ALTERNATIVE
BtaINT0165643
(ZNF513)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
chrUn_random:15070693-15070969 
Zebrafish
(danRer10)
chr17:5776696-5787513 
LOW PSI
DreINT0023142
(ZNF513 (2 of 2))
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CGGTTCGTGGATCCTAAGAGC

				
R: 
CTCTCAAAGCCCATGAGCTGG

				
Band lengths: 
304-552

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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