MmuINT1027484 @ mm10
Intron Retention
Gene
ENSMUSG00000061136 | Prpf40a
Description
pre-mRNA processing factor 40A [Source:MGI Symbol;Acc:MGI:1860512]
Coordinates
chr2:53145824-53146208:-
Coord C1 exon
chr2:53146094-53146208
Coord A exon
chr2:53145916-53146093
Coord C2 exon
chr2:53145824-53145915
Length
178 bp
Sequences
Splice sites
5' ss Seq
CGGGTGAGA
5' ss Score
7.62
3' ss Seq
TTCTTTAGTTTTTTCATTAGGAT
3' ss Score
6.29
Exon sequences
Seq C1 exon
ATCTTTCTGGACGAGTTGCACGAGCATGGGCAGCTGCACTCTATGTCCTCTTGGATGGAGCTGTATCCCACCATCAGCTCTGACATTCGCTTCACTAACATGCTGGGTCAGCCGG
Seq A exon
GTGAGATTCCTTTCTTTCCAAGCATACCTTAGGAGTTGGGATGGATGTGGGTTGGGGTTGGGAACAGCCGTTGGGGGAGGAGAGGTGTGTGCTTTATTTTATGTTTGTTTTACAAACAAAAAGTAATTGCAGCTCTTGGTTAGAGCTAGCTTTTTGACTTCTTTAGTTTTTTCATTAG
Seq C2 exon
GATCAACTGCACTTGATCTTTTCAAGTTTTATGTTGAAGATCTTAAAGCACGATATCATGATGAAAAGAAGATAATAAAAGACATTCTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000061136:ENSMUST00000076313:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184614=FF=FE(67.9=100)
A:
NA
C2:
PF0184614=FF=PD(17.9=32.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTTCTGGACGAGTTGCACG
R:
TCTTCTTTTCATCATGATATCGTGCT
Band lengths:
186-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types