RnoEX0051753 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141090651-141120063:-
Coord C1 exon
chr5:141118592-141120063
Coord A exon
chr5:141091019-141091180
Coord C2 exon
chr5:141090651-141090821
Length
162 bp
Sequences
Splice sites
3' ss Seq
TTTTCTTTGCAATTCTCCAGATG
3' ss Score
8.87
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GAGTGAACAGTAGCTGCCTGACCATGGAGAACAAGCTAGAGGGGATCGGGCAGTTTCACTGTCGAGTCCGAGAAATGTTTTCTCAATTGGCTGACCTGGATGATGAGCTAGATGGCATGGGAGCCATTGGTAGAGACACAGATAGCCTCCAGTCACAAATTGAGGACATACGGCTATTCCTTAACAAAATCCAAGCCCTCAGGCTTGACATAGAGGACTCTGAAGCAGAGTGTCGAAAAATGTTGGAGGAAGAGGGGACTTTAGACTTACTGGGTCTCAAGAGGGAGCTAGAAGCCCTTAACAAGCAGTGTGGTAAACTGACAGAGCGAGGGAAAGCTCGCCAGGAGCAGCTAGAGCTGACGCTGGGCCGAGTAGAGGACTTCTACAGTAAACTGAAAGCGCTCAATGATGCTGCCACTGCTGCAGAGGAGGGAGAAGCTCTTCAGTGGATTGTGGGGACTGAAGTCGATGTCATCAACCAGCAACTAGCAGATTTCAAA
Seq A exon
ATGTTTCAGAAAGAACAAGTGGATCCTCTTCAGGTGAAATTGCAGCAGGTGAATGGACTTGGCCAGGGATTAATTCAGAGTGCGGGAAAAACCTGTGATGTGCAGGGTTTAGAACATGACATGGAAGAGGTCAATACTCGATGGAATACACTGAATAAAAAG
Seq C2 exon
GTTGCACAAAGAATTGCACAACTGCAGGAGGCTTTGTTACATTGTGGGAAATTTCAAGATGCCTTAGAGCCATTGCTCAGCTGGTTGACAGACACCGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCCGAGTATAAAGTGGTGAAAGCCCAGATCCAAGAACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'86-98,'86-97,89-98
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.094 A=0.056 C2=0.018
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=WD(100=22.0),PF0043516=Spectrin=WD(100=21.4),PF0043516=Spectrin=WD(100=21.4),PF0043516=Spectrin=PU(39.8=8.8)
A:
PF0043516=Spectrin=FE(49.1=100)
C2:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAAGCGCTCAATGATGCTGC
R:
ATACTCGGCAGATGGAGGCTT
Band lengths:
244-406
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]