BtaEX6018685 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107234691-107265966:-
Coord C1 exon
chr3:107264495-107265966
Coord A exon
chr3:107235017-107235178
Coord C2 exon
chr3:107234691-107234861
Length
162 bp
Sequences
Splice sites
3' ss Seq
TTTGGCTTACAATTATCTAGATG
3' ss Score
4.97
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
GAGTGAACAGTGGCTGCATGGCCATGGAGAACAAGCTGGAGGGGATTGGCCAGTTTCACTGCCGCGTCCGAGAAATGTTTTCTCAGTTGGCAGACCTGGACGATGAGCTGGATGGCATGGGTGCTATCGGCCGAGACACTGACAGCCTCCAGTCCCAGATTGAGGACGTGCGGCTGTTCCTTAACAAGATCCAAGCCCTCAAGCTGGACATCGAGGCCTCAGAAGCAGAGTGCCGACAAATGCTGGAGGAAGAGGGGACTCTGGACCTGCTGGGTCTCAAGAGGGAGTTAGAAGCCCTGAGCAAACAGTGCAGCAAACTTACAGAGAGGAGCCGAGCTCGGCAGGAGCAGCTGGAGCTGACCCTGGGCCGCGTGGAGGACTTCTATCAGAAGCTGAAAGTGCTCAATGATATGACCACGGCAGCAGAGGAGGGGGAGGCCCTCCAGTGGGTCGTGGGGACCGAGGTGGATGTCATCAACCAACAATTAGCAGATTTTAAA
Seq A exon
ATGTTTCAGAAAGAACAGGTGGATCCTCTCCAGATGAAACTGCAACAGGTGAATGGACTTGGCCAGGGCTTGATTCAGAGCGCCGGAAAAAACTGTGATGTGCAGGGTTTGGAACATGACATGGAAGAAATCAACGCTCGGTGGAACACACTAAATAAAAAG
Seq C2 exon
GTTGCACAAAGAATCGCACAACTGCAAGAGGCTTTGTTGCATTGTGGGAAGTTCCAAGATGCCTTGGAGCCATTGCTCAGCTGGTTGGCAGACACTGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCTGAGTATAAAGTGGTGAAAGCCCAGATCCAAGAACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'67-71,'67-70,72-71=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.106 A=0.019 C2=0.018
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=WD(100=22.0),PF0043516=Spectrin=WD(100=21.4),PF0043516=Spectrin=PU(39.8=8.8)
A:
PF0043516=Spectrin=FE(49.1=100)
C2:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTACAGAGAGGAGCCGAGCT
R:
GGCATCTTGGAACTTCCCACA
Band lengths:
246-408
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]