DreEX0044244 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35497811-35529383:-
Coord C1 exon
chr19:35528883-35529383
Coord A exon
chr19:35498066-35498227
Coord C2 exon
chr19:35497811-35497981
Length
162 bp
Sequences
Splice sites
3' ss Seq
TTTCCCCACTCACTCTTTAGGCT
3' ss Score
10.98
5' ss Seq
AGGGTACGC
5' ss Score
8.18
Exon sequences
Seq C1 exon
CTGAGCAGTGCTGTTCATCATTGGAGTCCCGGTTGCAGGGTGTTGGAGAGGTCCAGTCGCGGGTCCGAGATGTTTTCTCTCGCCTGGCTGATTTGGATGATGAGCTGGACAGCCTGAGCCCTGTGGGACGGGATGCTGACTCTTTGGCTTCACAGGCTGATGCCGTCAGAGGCTTCCTGAGTCGACTCAATGCCCTGCGCTCAGAATTGGAGAATCATGGTGGAGAATGCACAACTATGCTGCGTCGAGAGGGCAGCTCTCCTGATCTTCTGGCTCTCAGGAGAGAGACCGAAGCCCTGAGCAGACAGGCAGGCAAGCTGGCAGAGCGAGGCCAGAACCGACTGGCACTCATAGAATCTGCAGAGGAGCGCGTGAGAGAGTTCTACGCCCGTCTGGCTGATCTGCAGGCGCTTCTGGGCCGGGCAGAGGAGGTGCTGAACACACAGGCTGTGGTGGGGACAGAAGTGGAGGTCATCAAACAGCAGCTTCAGGAGTTCAAG
Seq A exon
GCTGTGGAAAGGGAGCAGGTAGACAGCATTCAGCCTAAACTGCAACATGTCAACGCTGTGGGTCAAGGCCTTATTCAAAGCGCAGCCAAACACACTGACACTCAGGCTCTGGAACATGACTTGGAGACCACCAACCTGCACTGGAACTCCCTCAACAAGAGG
Seq C2 exon
GTTGCTGAAAGAATCGCACAGCTGCAAGAGGCCCTGTTGCATTGTGGGAAGTTTCAAGACGCCCTGGAGCCACTCTTGAGCTGGCTGAGTGACACAGAGGAACTGATAGCCAATCAGAAACCTCCATCTGCTGAGTACCGTGTTGTCAAGGCACAAATTCAGGAGCAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'69-73,'69-72,71-73
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.162 A=0.222 C2=0.035
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PU(40.4=26.3)
A:
PF0043516=Spectrin=FE(48.6=100)
C2:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCAGGAGAGAGACCGAAGC
R:
AGCTGTGCGATTCTTTCAGCA
Band lengths:
249-411
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]